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Journal of Virology, January 2000, p. 1038-1044, Vol. 74, No. 2
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The Human Herpesvirus 8 Homolog of Epstein-Barr Virus SM Protein (KS-SM) Is a Posttranscriptional Activator of Gene Expression

Ashish K. Gupta,1 Vivian Ruvolo,1 Cam Patterson,2 and Sankar Swaminathan1,3,*

Sealy Center for Oncology and Hematology,1 Sealy Center for Molecular Cardiology,2 and Division of Infectious Diseases, Department of Internal Medicine and Department of Microbiology and Immunology,3 University of Texas Medical Branch, Galveston, Texas 77555

Received 1 September 1999/Accepted 15 October 1999

Homologs of the Epstein-Barr virus (EBV) SM protein exist in several human and nonhuman herpesviruses. Structure and function differ significantly among these proteins. We have cloned and characterized the human herpesvirus 8 (HHV8) gene, KS-SM, which is homologous to the EBV SM and herpes simplex virus ICP27 genes, from an HHV8-infected primary effusion lymphoma. KS-SM is shown to be a posttranscriptional activator of gene expression in cotransfection studies. KS-SM activated gene expression in a gene-specific, promoter-independent manner. In particular, KS-SM enhanced the expression of KDR/flk-1, a receptor for vascular endothelial growth factor (VEGF), in cotransfection studies. Since expression of KDR/flk-1 is increased in Kaposi's sarcoma and HHV8-infected cell cultures and VEGF enhances the proliferation of HHV8-infected cells, KS-SM may play a pathogenic role in Kaposi's sarcoma.

* Corresponding author. Mailing address: Sealy Center for Oncology and Hematology, MRB 9.104, University of Texas Medical Branch, Galveston, TX 77555-1048. Phone: (409) 747-1935. Fax: (409) 747-1938. E-mail: sswamina{at}utmb.edu.

Journal of Virology, January 2000, p. 1038-1044, Vol. 74, No. 2
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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