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Journal of Virology, October 2000, p. 9078-9082, Vol. 74, No. 19
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Human Cytomegalovirus Virions Differentially Incorporate Viral and Host Cell RNA during the Assembly Process

Astrid E. Greijer,1,2 Chantal A. J. Dekkers,1 and Jaap M. Middeldorp1,2,*

Organon Teknika B.V., 5281 RM Boxtel,1 and Department of Pathology, Free University Hospital, 1081 HV Amsterdam,2 The Netherlands

Received 2 February 2000/Accepted 9 July 2000

While analyzing human cytomegalovirus (HCMV) gene expression in infected cells by RNA-specific nucleic acid sequence-based amplification (NASBA), positive results were observed for HCMV RNA encoded by several viral genes immediately after the addition of the virus. UV-inactivated virus also gave a positive NASBA result without establishing active infection, suggesting that RNA was associated with the inoculum. Highly purified virions devoid of cellular contamination proved to be positive for viral RNA encoding both immediate-early (UL123) and late (UL65) gene products. Virion-associated RNA might be incorporated specifically or without selection during the virion assembly. In the latter case, cellular RNA would also be present in the virion. A high-abundant cellular RNA encoded by GAPDH and even U1A RNA, which is expressed at low levels, were detected in the virion fraction, whereas cellular DNA was absent. Virion fractionation revealed that cellular RNA was absent in purified de-enveloped capsids. In conclusion, cellular and viral RNA was present between the capsid and envelope of the virion, whereas in the capsid only viral RNA could be detected. The results suggest that virion-associated viral and cellular RNA is incorporated nonspecifically during virion assembly.

* Corresponding author. Mailing address: Department of Pathology, Free University Hospital, 1081 HV Amsterdam, The Netherlands. Phone: 31-20-4444077. Fax: 31-20-4442964. E-mail: J.Middeldorp{at}azvu.nl.

Journal of Virology, October 2000, p. 9078-9082, Vol. 74, No. 19
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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