This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Coste, A.
Right arrow Articles by Kraehenbuhl, J.-P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Coste, A.
Right arrow Articles by Kraehenbuhl, J.-P.

 Previous Article  |  Next Article 

Journal of Virology, October 2000, p. 8966-8971, Vol. 74, No. 19
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Nasal Immunization of Mice with Virus-Like Particles Protects Offspring against Rotavirus Diarrhea

Alix Coste,1,2 Jean-Claude Sirard,1 Kari Johansen,3 Jean Cohen,4 and Jean-Pierre Kraehenbuhl1,*

Swiss Institute for Experimental Cancer Research and the Institute of Biochemistry, University of Lausanne, CH-1066 Epalinges, Switzerland1; Laboratoire de Microbiologie INRA, 63122 St. Genes-Champanelle,2 and Laboratoire de Virologie et d'Immunologie Moléculaire INRA, C.R.J. Domaine de Vilvert, 78350 Jouy-en-Josas,4 France; and Department of Virology, Smyttskyddsinstitutet, Karolinska Institute, 105 21 Stockholm, Sweden3

Received 6 April 2000/Accepted 13 July 2000

Rotavirus is the major cause of diarrhea among young infants in both humans and animals. Immune protection of newborns by vaccination is difficult to achieve since there is not enough time to mount an immune response before exposure to the virus. We have designed a vaccination strategy mediating transfer of neutralizing antibodies from the mother to the offspring during pregnancy and/or lactation. Adult female mice were nasally immunized with virus-like particles (VLPs) made of viral proteins VP2 and 6 (VLP2/6) or VP 2, 6, and 7 (VLP2/6/7) derived from the RF rotavirus strain in the presence or absence of cholera toxin. Both vaccines elicited serum and milk antibodies against the respective VPs. Four days after parturition, suckling pups were challenged orally with RF rotavirus. Pups from mothers immunized with VLP2/6/7 but not VLP2/6 were protected against rotavirus diarrhea, indicating that VP7 plays a key role in protection. Protection was mediated by milk rather than serum antibodies, and mucosal adjuvants were not required. In conclusion, VLPs containing VP7 administered nasally to mothers represent a promising vaccine candidate for the protection of suckling newborns against rotavirus-induced diarrhea, even in the absence of a mucosal adjuvant.

* Corresponding author. Mailing address: ISREC, CH-1066 Epalinges, Switzerland. Phone: (41 21) 692 58 56. Fax: (41 21) 652 69 33. E-mail: Jean-Pierre.Kraehenbuhl{at}isrec.unil.ch.

Journal of Virology, October 2000, p. 8966-8971, Vol. 74, No. 19
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Garaicoechea, L., Olichon, A., Marcoppido, G., Wigdorovitz, A., Mozgovoj, M., Saif, L., Surrey, T., Parreno, V. (2008). Llama-Derived Single-Chain Antibody Fragments Directed to Rotavirus VP6 Protein Possess Broad Neutralizing Activity In Vitro and Confer Protection against Diarrhea in Mice. J. Virol. 82: 9753-9764 [Abstract] [Full Text]  
  • Corthesy, B., Benureau, Y., Perrier, C., Fourgeux, C., Parez, N., Greenberg, H., Schwartz-Cornil, I. (2006). Rotavirus Anti-VP6 Secretory Immunoglobulin A Contributes to Protection via Intracellular Neutralization but Not via Immune Exclusion. J. Virol. 80: 10692-10699 [Abstract] [Full Text]  
  • Moret, Y. (2006). 'Trans-generational immune priming': specific enhancement of the antimicrobial immune response in the mealworm beetle, Tenebrio molitor. Proc R Soc B 273: 1399-1405 [Abstract] [Full Text]  
  • Fromantin, C., Jamot, B., Cohen, J., Piroth, L., Pothier, P., Kohli, E. (2001). Rotavirus 2/6 Virus-Like Particles Administered Intranasally in Mice, with or without the Mucosal Adjuvants Cholera Toxin and Escherichia coli Heat-Labile Toxin, Induce a Th1/Th2-Like Immune Response. J. Virol. 75: 11010-11016 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»