This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cromwell, M. A.
Right arrow Articles by Johnson, R. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cromwell, M. A.
Right arrow Articles by Johnson, R. P.

 Previous Article  |  Next Article 

Journal of Virology, September 2000, p. 8762-8766, Vol. 74, No. 18
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Induction of Mucosal Homing Virus-Specific CD8+ T Lymphocytes by Attenuated Simian Immunodeficiency Virus

Mandy A. Cromwell,1 Ronald S. Veazey,2 John D. Altman,3 Keith G. Mansfield,4 Rhona Glickman,1 Todd M. Allen,5 David I. Watkins,5 Andrew A. Lackner,2 and R. Paul Johnson1,6,*

Divisions of Immunology,1 Pathology,2 and Primate Medicine,4 New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772; Emory Vaccine Center at Yerkes, Emory University School of Medicine, Atlanta, Georgia 303223; Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, Wisconsin 537155; and Infectious Disease Unit and Partners AIDS Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 021156

Received 18 February 2000/Accepted 5 June 2000

Induction of virus-specific T-cell responses in mucosal as well as systemic compartments of the immune system is likely to be a critical feature of an effective AIDS vaccine. We investigated whether virus-specific CD8+ lymphocytes induced in rhesus macaques by immunization with attenuated simian immunodeficiency virus (SIV), an approach that is highly effective in eliciting protection against mucosal challenge, express the mucosa-homing receptor alpha 4beta 7 and traffic to the intestinal mucosa. SIV-specific CD8+ T cells expressing alpha 4beta 7 were detected in peripheral blood and intestine of macaques infected with attenuated SIV. In contrast, virus-specific T cells in blood of animals immunized cutaneously by a combined DNA-modified vaccinia virus Ankara regimen did not express alpha 4beta 7. These results demonstrate the selective induction of SIV-specific CD8+ T lymphocytes expressing alpha 4beta 7 by a vaccine approach that replicates in mucosal tissue and suggest that induction of virus-specific lymphocytes that are able to home to mucosal sites may be an important characteristic of a successful AIDS vaccine.

* Corresponding author. Mailing address: New England Regional Primate Research Center, Harvard Medical School, One Pine Hill Dr., P.O. Box 9102, Southborough, MA 01772-9102. Phone: (508) 624-8148. Fax: (508) 624-8172. E-mail: paul_johnson{at}hms.harvard.edu.

Journal of Virology, September 2000, p. 8762-8766, Vol. 74, No. 18
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sexton, A., De Rose, R., Reece, J. C., Alcantara, S., Loh, L., Moffat, J. M., Laurie, K., Hurt, A., Doherty, P. C., Turner, S. J., Kent, S. J., Stambas, J. (2009). Evaluation of Recombinant Influenza Virus-Simian Immunodeficiency Virus Vaccines in Macaques. J. Virol. 83: 7619-7628 [Abstract] [Full Text]  
  • Reynolds, M. R., Weiler, A. M., Weisgrau, K. L., Piaskowski, S. M., Furlott, J. R., Weinfurter, J. T., Kaizu, M., Soma, T., Leon, E. J., MacNair, C., Leaman, D. P., Zwick, M. B., Gostick, E., Musani, S. K., Price, D. A., Friedrich, T. C., Rakasz, E. G., Wilson, N. A., McDermott, A. B., Boyle, R., Allison, D. B., Burton, D. R., Koff, W. C., Watkins, D. I. (2008). Macaques vaccinated with live-attenuated SIV control replication of heterologous virus. JEM 205: 2537-2550 [Abstract] [Full Text]  
  • Belyakov, I. M., Isakov, D., Zhu, Q., Dzutsev, A., Berzofsky, J. A. (2007). A Novel Functional CTL Avidity/Activity Compartmentalization to the Site of Mucosal Immunization Contributes to Protection of Macaques against Simian/Human Immunodeficiency Viral Depletion of Mucosal CD4+ T Cells. J. Immunol. 178: 7211-7221 [Abstract] [Full Text]  
  • Woodberry, T., Suscovich, T. J., Henry, L. M., August, M., Waring, M. T., Kaur, A., Hess, C., Kutok, J. L., Aster, J. C., Wang, F., Scadden, D. T., Brander, C. (2005). {alpha}E{beta}7 (CD103) Expression Identifies a Highly Active, Tonsil-Resident Effector-Memory CTL Population. J. Immunol. 175: 4355-4362 [Abstract] [Full Text]  
  • Bertley, F. M. N., Kozlowski, P. A., Wang, S.-W., Chappelle, J., Patel, J., Sonuyi, O., Mazzara, G., Montefiori, D., Carville, A., Mansfield, K. G., Aldovini, A. (2004). Control of Simian/Human Immunodeficiency Virus Viremia and Disease Progression after IL-2-Augmented DNA-Modified Vaccinia Virus Ankara Nasal Vaccination in Nonhuman Primates. J. Immunol. 172: 3745-3757 [Abstract] [Full Text]  
  • Evans, D. T., Chen, L.-M., Gillis, J., Lin, K.-C., Harty, B., Mazzara, G. P., Donis, R. O., Mansfield, K. G., Lifson, J. D., Desrosiers, R. C., Galan, J. E., Johnson, R. P. (2003). Mucosal Priming of Simian Immunodeficiency Virus-Specific Cytotoxic T-Lymphocyte Responses in Rhesus Macaques by the Salmonella Type III Secretion Antigen Delivery System. J. Virol. 77: 2400-2409 [Abstract] [Full Text]  
  • Stevceva, L., Alvarez, X., Lackner, A. A., Tryniszewska, E., Kelsall, B., Nacsa, J., Tartaglia, J., Strober, W., Franchini, G. (2002). Both Mucosal and Systemic Routes of Immunization with the Live, Attenuated NYVAC/Simian Immunodeficiency Virus SIVgpe Recombinant Vaccine Result in Gag-Specific CD8+ T-Cell Responses in Mucosal Tissues of Macaques. J. Virol. 76: 11659-11676 [Abstract] [Full Text]  
  • Allen, T. M., Jing, P., Calore, B., Horton, H., O'Connor, D. H., Hanke, T., Piekarczyk, M., Ruddersdorf, R., Mothe, B. R., Emerson, C., Wilson, N., Lifson, J. D., Belyakov, I. M., Berzofsky, J. A., Wang, C., Allison, D. B., Montefiori, D. C., Desrosiers, R. C., Wolinsky, S., Kunstman, K. J., Altman, J. D., Sette, A., McMichael, A. J., Watkins, D. I. (2002). Effects of Cytotoxic T Lymphocytes (CTL) Directed against a Single Simian Immunodeficiency Virus (SIV) Gag CTL Epitope on the Course of SIVmac239 Infection. J. Virol. 76: 10507-10511 [Abstract] [Full Text]  
  • Stevceva, L., Kelsall, B., Nacsa, J., Moniuszko, M., Hel, Z., Tryniszewska, E., Franchini, G. (2002). Cervicovaginal Lamina Propria Lymphocytes: Phenotypic Characterization and Their Importance in Cytotoxic T-Lymphocyte Responses to Simian Immunodeficiency Virus SIVmac251. J. Virol. 76: 9-18 [Abstract] [Full Text]  
  • Ferko, B., Stasakova, J., Sereinig, S., Romanova, J., Katinger, D., Niebler, B., Katinger, H., Egorov, A. (2001). Hyperattenuated Recombinant Influenza A Virus Nonstructural-Protein-Encoding Vectors Induce Human Immunodeficiency Virus Type 1 Nef-Specific Systemic and Mucosal Immune Responses in Mice. J. Virol. 75: 8899-8908 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»