Localization of Human Immunodeficiency Virus Type 1 Gag and Env at the Plasma Membrane by Confocal Imaging

doi:10.1128/JVI.74.18.8670-8679.2000

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Journal of Virology, September 2000, p. 8670-8679, Vol. 74, No. 18
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Localization of Human Immunodeficiency Virus Type 1 Gag and Env at the Plasma Membrane by Confocal Imaging

Luz Hermida-Matsumoto and Marilyn D. Resh^*

Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021

Received 5 April 2000/Accepted 20 June 2000

Budding of lentiviruses occurs at the plasma membrane, but the preceding steps involved in particle assembly are poorly understood.Since the Gag polyprotein mediates virion assembly and budding,studies on the localization of Gag within the cell should provideinsight into the mechanism of particle assembly. Here, we utilizebiochemical fractionation techniques as well as high-resolutionconfocal imaging of live cells to demonstrate that Gag is localizedat the plasma membrane in a striking punctate pattern. Mutationof the N-terminal myristoylation site results in the formationof large cytosolic complexes, whereas mutation of the N-terminalbasic residue cluster in the matrix domain redirects the Gag proteinto a region partially overlapping the Golgi apparatus. In addition,we show that Gag and Env colocalize at the plasma membrane andthat mistargeting of a mutant Gag to the Golgi apparatus altersthe pattern of surface expression ofEnv.

^* Corresponding author. Mailing address: Memorial Sloan-Kettering Cancer Center, 1275 York Ave., Box 143, New York, NY 10021.Phone: (212) 639-2514. Fax: (212) 717-3317. E-mail: m-resh{at}ski.mskcc.org.

Journal of Virology, September 2000, p. 8670-8679, Vol. 74, No. 18
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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