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Journal of Virology, September 2000, p. 8582-8588, Vol. 74, No. 18
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Early Steps of Polyomavirus Entry into Cells

Joanna M. Gilbert* and Thomas L. Benjamin

Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115

Received 24 May 2000/Accepted 26 June 2000

The mechanism by which murine polyomavirus penetrates cells and arrives at the nucleus, the site of viral replication, is not well understood. Simian virus 40 and JC virus, two closely related members of the polyomavirus subfamily, use caveola- and clathrin-mediated uptake pathways for entry, respectively. The data presented here indicate that compounds that block endocytosis of both caveola- and clathrin-derived vesicles have no effect on polyomavirus infectivity. Polyomavirus does not appear to colocalize with either clathrin light chain or caveolin-1 by immunofluorescence microscopy. Additionally, expression of a dominant-negative form of dynamin I has no effect on polyomavirus uptake and infectivity. Therefore, polyomavirus uptake occurs through a class of uncoated vesicles in a clathrin-, caveolin-1-, and dynamin I-independent manner.

* Corresponding author. Mailing address: Harvard Medical School Department of Pathology, 200 Longwood Ave., Armenise-233, Boston, MA 02115. Phone: (617) 432-1998. Fax: (617) 277-5291. E-mail: jgilbert{at}hms.harvard.edu.

Journal of Virology, September 2000, p. 8582-8588, Vol. 74, No. 18
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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