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Journal of Virology, September 2000, p. 8582-8588, Vol. 74, No. 18
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Early Steps of Polyomavirus Entry into
Cells
Joanna M.
Gilbert* and
Thomas L.
Benjamin
Department of Pathology, Harvard Medical
School, Boston, Massachusetts 02115
Received 24 May 2000/Accepted 26 June 2000
The mechanism by which murine polyomavirus penetrates cells and
arrives at the nucleus, the site of viral replication, is not well
understood. Simian virus 40 and JC virus, two closely related members
of the polyomavirus subfamily, use caveola- and clathrin-mediated
uptake pathways for entry, respectively. The data presented here
indicate that compounds that block endocytosis of both caveola- and
clathrin-derived vesicles have no effect on polyomavirus infectivity.
Polyomavirus does not appear to colocalize with either clathrin light
chain or caveolin-1 by immunofluorescence microscopy. Additionally,
expression of a dominant-negative form of dynamin I has no effect on
polyomavirus uptake and infectivity. Therefore, polyomavirus uptake
occurs through a class of uncoated vesicles in a clathrin-,
caveolin-1-, and dynamin I-independent manner.
*
Corresponding author. Mailing address: Harvard Medical
School Department of Pathology, 200 Longwood Ave., Armenise-233,
Boston, MA 02115. Phone: (617) 432-1998. Fax: (617) 277-5291. E-mail: jgilbert{at}hms.harvard.edu.
Journal of Virology, September 2000, p. 8582-8588, Vol. 74, No. 18
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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