This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gilbert, J. M.
Right arrow Articles by Benjamin, T. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gilbert, J. M.
Right arrow Articles by Benjamin, T. L.

 Previous Article  |  Next Article 

Journal of Virology, September 2000, p. 8582-8588, Vol. 74, No. 18
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Early Steps of Polyomavirus Entry into Cells

Joanna M. Gilbert* and Thomas L. Benjamin

Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115

Received 24 May 2000/Accepted 26 June 2000

The mechanism by which murine polyomavirus penetrates cells and arrives at the nucleus, the site of viral replication, is not well understood. Simian virus 40 and JC virus, two closely related members of the polyomavirus subfamily, use caveola- and clathrin-mediated uptake pathways for entry, respectively. The data presented here indicate that compounds that block endocytosis of both caveola- and clathrin-derived vesicles have no effect on polyomavirus infectivity. Polyomavirus does not appear to colocalize with either clathrin light chain or caveolin-1 by immunofluorescence microscopy. Additionally, expression of a dominant-negative form of dynamin I has no effect on polyomavirus uptake and infectivity. Therefore, polyomavirus uptake occurs through a class of uncoated vesicles in a clathrin-, caveolin-1-, and dynamin I-independent manner.

* Corresponding author. Mailing address: Harvard Medical School Department of Pathology, 200 Longwood Ave., Armenise-233, Boston, MA 02115. Phone: (617) 432-1998. Fax: (617) 277-5291. E-mail: jgilbert{at}hms.harvard.edu.

Journal of Virology, September 2000, p. 8582-8588, Vol. 74, No. 18
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Jiang, M., Abend, J. R., Tsai, B., Imperiale, M. J. (2009). Early Events during BK Virus Entry and Disassembly. J. Virol. 83: 1350-1358 [Abstract] [Full Text]  
  • Dewerchin, H. L., Cornelissen, E., Van Hamme, E., Smits, K., Verhasselt, B., Nauwynck, H. J. (2008). Surface-expressed viral proteins in feline infectious peritonitis virus-infected monocytes are internalized through a clathrin- and caveolae-independent pathway. J. Gen. Virol. 89: 2731-2740 [Abstract] [Full Text]  
  • Cantin, C., Holguera, J., Ferreira, L., Villar, E., Munoz-Barroso, I. (2007). Newcastle disease virus may enter cells by caveolae-mediated endocytosis. J. Gen. Virol. 88: 559-569 [Abstract] [Full Text]  
  • Lilley, B. N., Gilbert, J. M., Ploegh, H. L., Benjamin, T. L. (2006). Murine polyomavirus requires the endoplasmic reticulum protein derlin-2 to initiate infection.. J. Virol. 80: 8739-8744 [Abstract] [Full Text]  
  • Liebl, D., Difato, F., Hornikova, L., Mannova, P., Stokrova, J., Forstova, J. (2006). Mouse Polyomavirus Enters Early Endosomes, Requires Their Acidic pH for Productive Infection, and Meets Transferrin Cargo in Rab11-Positive Endosomes. J. Virol. 80: 4610-4622 [Abstract] [Full Text]  
  • Dahl, J., You, J., Benjamin, T. L. (2005). Induction and Utilization of an ATM Signaling Pathway by Polyomavirus. J. Virol. 79: 13007-13017 [Abstract] [Full Text]  
  • Eash, S., Atwood, W. J. (2005). Involvement of Cytoskeletal Components in BK Virus Infectious Entry. J. Virol. 79: 11734-11741 [Abstract] [Full Text]  
  • Berryman, S., Clark, S., Monaghan, P., Jackson, T. (2005). Early Events in Integrin {alpha}v{beta}6-Mediated Cell Entry of Foot-and-Mouth Disease Virus. J. Virol. 79: 8519-8534 [Abstract] [Full Text]  
  • Bossis, I., Roden, R. B. S., Gambhira, R., Yang, R., Tagaya, M., Howley, P. M., Meneses, P. I. (2005). Interaction of tSNARE Syntaxin 18 with the Papillomavirus Minor Capsid Protein Mediates Infection. J. Virol. 79: 6723-6731 [Abstract] [Full Text]  
  • Damm, E.-M., Pelkmans, L., Kartenbeck, J., Mezzacasa, A., Kurzchalia, T., Helenius, A. (2005). Clathrin- and caveolin-1-independent endocytosis: entry of simian virus 40 into cells devoid of caveolae. JCB 168: 477-488 [Abstract] [Full Text]  
  • Gilbert, J., Dahl, J., Riney, C., You, J., Cui, C., Holmes, R., Lencer, W., Benjamin, T. (2005). Ganglioside GD1a Restores Infectibility to Mouse Cells Lacking Functional Receptors for Polyomavirus. J. Virol. 79: 615-618 [Abstract] [Full Text]  
  • Gilbert, J., Benjamin, T. (2004). Uptake Pathway of Polyomavirus via Ganglioside GD1a. J. Virol. 78: 12259-12267 [Abstract] [Full Text]  
  • Tian, Y., Li, D., Dahl, J., You, J., Benjamin, T. (2004). Identification of TAZ as a Binding Partner of the Polyomavirus T Antigens. J. Virol. 78: 12657-12664 [Abstract] [Full Text]  
  • Eash, S., Querbes, W., Atwood, W. J. (2004). Infection of Vero Cells by BK Virus Is Dependent on Caveolae. J. Virol. 78: 11583-11590 [Abstract] [Full Text]  
  • Sanchez-San Martin, C., Lopez, T., Arias, C. F., Lopez, S. (2004). Characterization of Rotavirus Cell Entry. J. Virol. 78: 2310-2318 [Abstract] [Full Text]  
  • Querbes, W., Benmerah, A., Tosoni, D., Di Fiore, P. P., Atwood, W. J. (2004). A JC Virus-Induced Signal Is Required for Infection of Glial Cells by a Clathrin- and eps15-Dependent Pathway. J. Virol. 78: 250-256 [Abstract] [Full Text]  
  • Shin, Y. C., Folk, W. R. (2003). Formation of Polyomavirus-Like Particles with Different VP1 Molecules That Bind the Urokinase Plasminogen Activator Receptor. J. Virol. 77: 11491-11498 [Abstract] [Full Text]  
  • Caruso, M., Belloni, L., Sthandier, O., Amati, P., Garcia, M.-I. (2003). {alpha}4{beta}1 Integrin Acts as a Cell Receptor for Murine Polyomavirus at the Postattachment Level. J. Virol. 77: 3913-3921 [Abstract] [Full Text]  
  • Gilbert, J. M., Goldberg, I. G., Benjamin, T. L. (2003). Cell Penetration and Trafficking of Polyomavirus. J. Virol. 77: 2615-2622 [Abstract] [Full Text]  
  • Mannova, P., Forstova, J. (2003). Mouse Polyomavirus Utilizes Recycling Endosomes for a Traffic Pathway Independent of COPI Vesicle Transport. J. Virol. 77: 1672-1681 [Abstract] [Full Text]  
  • Stuart, A. D., Eustace, H. E., McKee, T. A., Brown, T. D. K. (2002). A Novel Cell Entry Pathway for a DAF-Using Human Enterovirus Is Dependent on Lipid Rafts. J. Virol. 76: 9307-9322 [Abstract] [Full Text]  
  • Sieczkarski, S. B., Whittaker, G. R. (2002). Dissecting virus entry via endocytosis. J. Gen. Virol. 83: 1535-1545 [Abstract] [Full Text]  
  • Richterova, Z., Liebl, D., Horak, M., Palkova, Z., Hozak, P., Korb, J., Forstova, J. (2001). Caveolae Are Involved in the Trafficking of Mouse Polyomavirus Virions and Artificial VP1 Pseudocapsids toward Cell Nuclei. J. Virol. 75: 10880-10891 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»