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Journal of Virology, September 2000, p. 8558-8562, Vol. 74, No. 18
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Interaction of Cellular Proteins with the 5' End of Norwalk Virus Genomic RNA

Ana Lorena Gutiérrez-Escolano,1,* Zamirath Uribe Brito,1 Rosa M. del Angel,1 and Xi Jiang2

Departamento de Patología Experimental, Centro de Investigación y de Estudios Avanzados del IPN, Mexico City, Mexico,1 and Center for Pediatric Research, Children's Hospital for the King's Daughters, Eastern Virginia Medical School, Norfolk, Virginia2

Received 29 February 2000/Accepted 20 June 2000

The lack of a susceptible cell line and an animal model for Norwalk virus (NV) infection has prompted the development of alternative strategies to generate in vitro RNAs that approximate the authentic viral genome. This approach has allowed the study of viral RNA replication and gene expression. In this study, using mobility shift and cross-linking assays, we detected several cellular proteins from HeLa and CaCo-2 cell extracts that bind to, and form stable complexes with, the first 110 nucleotides of the 5' end of NV genomic RNA, a region previously predicted to form a double stem-loop structure. These proteins had molecular weights similar to those of the HeLa cellular proteins that bind to the internal ribosomal entry site of poliovirus RNA. HeLa proteins La, PCBP-2, and PTB, which are important for poliovirus translation, and hnRNP L, which is possibly implicated in hepatitis C virus translation, interact with NV RNA. These protein-RNA interactions are likely to play a role in NV translation and/or replication.

* Corresponding author. Mailing address: Departamento de Patología Experimental, Centro de Investigación y de Estudios Avanzados del IPN, Av. IPN 2508, Col. San Pedro Zacatenco, México, D.F. C.P. 07360, México. Phone: (52)5 747-3800, ext. 5647. Fax: (52)5 747-7107. E-mail: alonso{at}mail.cinvestav.mx.

Journal of Virology, September 2000, p. 8558-8562, Vol. 74, No. 18
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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