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Journal of Virology, September 2000, p. 8119-8126, Vol. 74, No. 17
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Mink Cell Focus-Forming Murine Leukemia Virus Infection Induces Apoptosis of Thymic Lymphocytes

Fayth K. Yoshimura,1,* Tao Wang,1 Fei Yu,2 Hyeong-Reh C. Kim,2 and Jerrold R. Turner2

Department of Immunology and Microbiology and the Karmanos Cancer Institute1 and Department of Pathology,2 Wayne State University, Detroit, Michigan 48201

Received 9 February 2000/Accepted 1 June 2000

In a previous study we identified the subpopulations of thymus cells that were infected by the lymphomagenic MCF13 murine leukemia virus (MLV) (F. K. Yoshimura, T. Wang, and M. Cankovic, J. Virol. 73:4890-4898, 1999) and observed an effect on thymus size by virus infection. In this report we describe our results which demonstrate that MCF13 MLV infection of thymuses reduced the number of T lymphocytes in this organ. Histological examination showed diffuse lymphocyte depletion, which was most striking in the CD4+ CD8+ lymphocyte-enriched cortical zone. Consistent with this, flow cytometric analysis showed that the lymphocytes which were depleted were predominantly the immature CD3- CD4+ CD8+ and CD3+ CD4+ CD8+ cells. A comparison of the percentages of live, apoptotic, and dead cells of the gp70+ and gp70- thymic lymphocytes suggested that this effect on thymus cellularity is a result of virus infection. Studies of the survival of thymic T lymphocytes in culture showed that cells from MCF13 MLV-inoculated mice underwent greater apoptosis and death than cells from control animals. Assays for apoptosis included 7-amino-actinomycin D staining, DNA fragmentation, and cleavage of caspase-3 and poly(ADP-ribose) polymerase proenzymes. Our results suggest that apoptosis of thymic lymphocytes by virus infection is an important step in the early stages of MCF13 MLV tumorigenesis.

* Corresponding author. Mailing address: Department of Immunology and Microbiology, Wayne State University, 540 E. Canfield, Detroit, MI 48201. Phone: (313) 577-1571. Fax: (313) 577-1155. E-mail: fyoshi{at}med.wayne.edu.

Journal of Virology, September 2000, p. 8119-8126, Vol. 74, No. 17
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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