This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Roberts, T. M.
Right arrow Articles by Boris-Lawrie, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Roberts, T. M.
Right arrow Articles by Boris-Lawrie, K.

 Previous Article  |  Next Article 

Journal of Virology, September 2000, p. 8111-8118, Vol. 74, No. 17
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The 5' RNA Terminus of Spleen Necrosis Virus Stimulates Translation of Nonviral mRNA

Tiffiney M. Roberts1,2,3,4 and Kathleen Boris-Lawrie1,2,3,4,5,*

Center for Retrovirus Research,1 Departments of Veterinary Biosciences2 and of Molecular Virology, Immunology,5 and Medical Genetics, Comprehensive Cancer Center,3 and Molecular, Cellular, and Developmental Biology Graduate Program,4 The Ohio State University, Columbus, Ohio 43210-1093

Received 13 December 1999/Accepted 7 June 2000

The RU5 region at the 5' RNA terminus of spleen necrosis virus (SNV) has been shown to facilitate expression of human immunodeficiency virus type 1 (HIV) unspliced RNA independently of the Rev-responsive element (RRE) and Rev. The SNV sequences act as a distinct posttranscriptional control element to stimulate gag RNA nuclear export and association with polyribosomes. Here we sought to determine whether RU5 functions to neutralize the cis-acting inhibitory sequences (INSs) in HIV RNA that confer RRE/Rev dependence or functions as an independent stimulatory sequence. Experiments with HIV gag reporter plasmids that contain inactivated INS-1 indicated that neutralization of INSs does not account for RU5 function. Results with luciferase reporter gene (luc) plasmids further indicated that RU5 stimulates expression of a nonretroviral RNA that lacks INSs. Northern blot and RT-PCR analyses indicated that RU5 does not increase the steady-state levels or nuclear export of the luc transcript but rather that the U5 region facilitates efficient polyribosomal association of the mRNA. RU5 does not function as an internal ribosome entry site in bicistronic reporter plasmids, and it requires the 5'-proximal position for efficient function. Our results indicate that RU5 contains stimulatory sequences that function in a 5'-proximal position to enhance initiation of translation of a nonretroviral reporter gene RNA. We speculate that RU5 evolved to overcome the translation-inhibitory effect of the highly structured encapsidation signal and other replication motifs in the 5' untranslated region of the retroviral RNA.

* Corresponding author. Mailing address: Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University Comprehensive Cancer Center, 1925 Coffey Rd., Columbus, OH 43210-1093. Phone: (614) 292-1392. Fax: (614) 292-6473. E-mail: boris-lawrie.1{at}osu.edu.

Journal of Virology, September 2000, p. 8111-8118, Vol. 74, No. 17
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Groom, H. C. T., Anderson, E. C., Lever, A. M. L. (2009). Rev: beyond nuclear export. J. Gen. Virol. 90: 1303-1318 [Abstract] [Full Text]  
  • Bolinger, C., Yilmaz, A., Hartman, T. R., Kovacic, M. B., Fernandez, S., Ye, J., Forget, M., Green, P. L., Boris-Lawrie, K. (2007). RNA helicase A interacts with divergent lymphotropic retroviruses and promotes translation of human T-cell leukemia virus type 1. Nucleic Acids Res 0: gkm124v1-14 [Abstract] [Full Text]  
  • Kraunus, J., Zychlinski, D., Heise, T., Galla, M., Bohne, J., Baum, C. (2006). Murine Leukemia Virus Regulates Alternative Splicing through Sequences Upstream of the 5{middle dot} Splice Site. J. Biol. Chem. 281: 37381-37390 [Abstract] [Full Text]  
  • Roberts, T. M., Boris-Lawrie, K. (2003). Primary Sequence and Secondary Structure Motifs in Spleen Necrosis Virus RU5 Confer Translational Utilization of Unspliced Human Immunodeficiency Virus Type 1 Reporter RNA. J. Virol. 77: 11973-11984 [Abstract] [Full Text]  
  • Hull, S., Boris-Lawrie, K. (2002). RU5 of Mason-Pfizer Monkey Virus 5' Long Terminal Repeat Enhances Cytoplasmic Expression of Human Immunodeficiency Virus Type 1 gag-pol and Nonviral Reporter RNA. J. Virol. 76: 10211-10218 [Abstract] [Full Text]  
  • Short, J. D., Pfarr, C. M. (2002). Translational Regulation of the JunD Messenger RNA. J. Biol. Chem. 277: 32697-32705 [Abstract] [Full Text]  
  • Dangel, A. W., Hull, S., Roberts, T. M., Boris-Lawrie, K. (2002). Nuclear Interactions Are Necessary for Translational Enhancement by Spleen Necrosis Virus RU5. J. Virol. 76: 3292-3300 [Abstract] [Full Text]  
  • Russell, R. A., Zeng, Y., Erlwein, O., Cullen, B. R., McClure, M. O. (2001). The R Region Found in the Human Foamy Virus Long Terminal Repeat Is Critical for both Gag and Pol Protein Expression. J. Virol. 75: 6817-6824 [Abstract] [Full Text]  
  • Butsch, M., Boris-Lawrie, K. (2000). Translation Is Not Required To Generate Virion Precursor RNA in Human Immunodeficiency Virus Type 1-Infected T Cells. J. Virol. 74: 11531-11537 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»