This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kacani, L.
Right arrow Articles by Dierich, M. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kacani, L.
Right arrow Articles by Dierich, M. P.

 Previous Article  |  Next Article 

Journal of Virology, September 2000, p. 7997-8002, Vol. 74, No. 17
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Detachment of Human Immunodeficiency Virus Type 1 from Germinal Centers by Blocking Complement Receptor Type 2

Laco Kacani,1,2 Wolfgang M. Prodinger,1,* Georg M. Sprinzl,3 Michael G. Schwendinger,1,dagger Martin Spruth,1,2,dagger Heribert Stoiber,1,2 Susanne Döpper,1,2 Sabine Steinhuber,1 Franz Steindl,4 and Manfred P. Dierich1,2

Ludwig Boltzmann Institute for AIDS Research,2 Institute for Hygiene and Social Medicine,1 and Department of Otorhinolaryngology,3 University of Innsbruck, A-6020 Innsbruck, and Institute of Applied Microbiology, University of Agriculture, A-1190 Vienna,4 Austria

Received 17 February 2000/Accepted 9 June 2000

After the transition from the acute to the chronic phase of human immunodeficiency virus (HIV) infection, complement mediates long-term storage of virions in germinal centers (GC) of lymphoid tissue. The contribution of particular complement receptors (CRs) to virus trapping in GC was studied on tonsillar specimens from HIV-infected individuals. CR2 (CD21) was identified as the main binding site for HIV in GC. Monoclonal antibodies (MAb) blocking the CR2-C3d interaction were shown to detach 62 to 77% of HIV type 1 from tonsillar cells of an individual in the presymptomatic stage. Although they did so at a lower efficiency, these antibodies were able to remove HIV from tonsillar cells of patients under highly active antiretroviral therapy, suggesting that the C3d-CR2 interaction remains a primary entrapment mechanism in treated patients as well. In contrast, removal of HIV was not observed with MAb blocking CR1 or CR3. Thus, targeting CR2 may facilitate new approaches toward a reduction of residual virus in GC.

* Corresponding author. Mailing address: Institut für Hygiene und Sozialmedizin, Universität Innsbruck, Fritz-Pregl-Strasse 3, A-6020 Innsbruck, Austria. Phone: 43 512 507 3424. Fax: 43 512 507 2870. E-mail: wolfgang.prodinger{at}uibk.ac.at.

dagger Present address: Baxter AG, A-1221 Vienna, Austria.

Journal of Virology, September 2000, p. 7997-8002, Vol. 74, No. 17
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Gaufin, T., Gautam, R., Kasheta, M., Ribeiro, R., Ribka, E., Barnes, M., Pattison, M., Tatum, C., MacFarland, J., Montefiori, D., Kaur, A., Pandrea, I., Apetrei, C. (2009). Limited ability of humoral immune responses in control of viremia during infection with SIVsmmD215 strain. Blood 113: 4250-4261 [Abstract] [Full Text]  
  • Moir, S., Ho, J., Malaspina, A., Wang, W., DiPoto, A. C., O'Shea, M. A., Roby, G., Kottilil, S., Arthos, J., Proschan, M. A., Chun, T.-W., Fauci, A. S. (2008). Evidence for HIV-associated B cell exhaustion in a dysfunctional memory B cell compartment in HIV-infected viremic individuals. JEM 205: 1797-1805 [Abstract] [Full Text]  
  • Keele, B. F., Tazi, L., Gartner, S., Liu, Y., Burgon, T. B., Estes, J. D., Thacker, T. C., Crandall, K. A., McArthur, J. C., Burton, G. F. (2008). Characterization of the Follicular Dendritic Cell Reservoir of Human Immunodeficiency Virus Type 1. J. Virol. 82: 5548-5561 [Abstract] [Full Text]  
  • Ho, J., Moir, S., Kulik, L., Malaspina, A., Donoghue, E. T., Miller, N. J., Wang, W., Chun, T.-W., Fauci, A. S., Holers, V. M. (2007). Role for CD21 in the Establishment of an Extracellular HIV Reservoir in Lymphoid Tissues. J. Immunol. 178: 6968-6974 [Abstract] [Full Text]  
  • Bouhlal, H., Chomont, N., Requena, M., Nasreddine, N., Saidi, H., Legoff, J., Kazatchkine, M. D., Belec, L., Hocini, H. (2007). Opsonization of HIV with Complement Enhances Infection of Dendritic Cells and Viral Transfer to CD4 T Cells in a CR3 and DC-SIGN-Dependent Manner. J. Immunol. 178: 1086-1095 [Abstract] [Full Text]  
  • Banki, Z., Wilflingseder, D., Ammann, C. G., Pruenster, M., Mullauer, B., Hollander, K., Meyer, M., Sprinzl, G. M., van Lunzen, J., Stellbrink, H.-J., Dierich, M. P., Stoiber, H. (2006). Factor I-Mediated Processing of Complement Fragments on HIV Immune Complexes Targets HIV to CR2-Expressing B Cells and Facilitates B Cell-Mediated Transmission of Opsonized HIV to T Cells.. J. Immunol. 177: 3469-3476 [Abstract] [Full Text]  
  • Lindorfer, M. A., Jinivizian, H. B., Foley, P. L., Kennedy, A. D., Solga, M. D., Taylor, R. P. (2003). B Cell Complement Receptor 2 Transfer Reaction. J. Immunol. 170: 3671-3678 [Abstract] [Full Text]  
  • Malaspina, A., Moir, S., Nickle, D. C., Donoghue, E. T., Ogwaro, K. M., Ehler, L. A., Liu, S., Mican, J. A. M., Dybul, M., Chun, T.-W., Mullins, J. I., Fauci, A. S. (2002). Human Immunodeficiency Virus Type 1 Bound to B Cells: Relationship to Virus Replicating in CD4+ T Cells and Circulating in Plasma. J. Virol. 76: 8855-8863 [Abstract] [Full Text]  
  • Smith-Franklin, B. A., Keele, B. F., Tew, J. G., Gartner, S., Szakal, A. K., Estes, J. D., Thacker, T. C., Burton, G. F. (2002). Follicular Dendritic Cells and the Persistence of HIV Infectivity: The Role of Antibodies and Fc{gamma} Receptors. J. Immunol. 168: 2408-2414 [Abstract] [Full Text]  
  • Guthridge, J. M., Young, K., Gipson, M. G., Sarrias, M.-R., Szakonyi, G., Chen, X. S., Malaspina, A., Donoghue, E., James, J. A., Lambris, J. D., Moir, S. A., Perkins, S. J., Holers, V. M. (2001). Epitope Mapping Using the X-Ray Crystallographic Structure of Complement Receptor Type 2 (CR2)/CD21: Identification of a Highly Inhibitory Monoclonal Antibody That Directly Recognizes the CR2-C3d Interface. J. Immunol. 167: 5758-5766 [Abstract] [Full Text]  
  • Smith, B. A., Gartner, S., Liu, Y., Perelson, A. S., Stilianakis, N. I., Keele, B. F., Kerkering, T. M., Ferreira-Gonzalez, A., Szakal, A. K., Tew, J. G., Burton, G. F. (2001). Persistence of Infectious HIV on Follicular Dendritic Cells. J. Immunol. 166: 690-696 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»