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Journal of Virology, September 2000, p. 7787-7793, Vol. 74, No. 17
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

DNA Vaccines for Influenza Virus: Differential Effects of Maternal Antibody on Immune Responses to Hemagglutinin and Nucleoprotein

Tamera M. Pertmer, Alp E. Oran, Janice M. Moser, Catherine A. Madorin, and Harriet L. Robinson*

Division of Microbiology and Immunology, Yerkes Primate Research Center, Emory University, Atlanta, Georgia 30329

Received 28 December 1999/Accepted 26 May 2000

Maternal antibody is the major form of protection from disease in early life when the neonatal immune system is still immature; however, the presence of maternal antibody also interferes with active immunization, placing infants at risk for severe bacterial and viral infection. We tested the ability of intramuscular and gene gun immunization with DNA expressing influenza virus hemagglutinin (HA) and nucleoprotein (NP) to raise protective humoral and cellular responses in the presence or absence of maternal antibody. Neonatal mice born to influenza virus-immune mothers raised full antibody responses to NP but failed to generate antibody responses to HA. In contrast, the presence of maternal antibody did not affect the generation of long-lived CD8+ T-cell responses to both HA and NP. Thus, maternal antibody did not affect cell-mediated responses but did affect humoral responses, with the ability to limit the antibody response correlating with whether the DNA-expressed immunogen was localized in the plasma membrane or within the cell.

* Corresponding author. Mailing address: Division of Microbiology and Immunology, Yerkes Primate Research Center, Emory University, 954 Gatewood Road NE, Atlanta, GA 30329. Phone: (404) 727-7377. Fax: (404) 727-7768. E-mail: hrobins{at}rmy.emory.edu.

Journal of Virology, September 2000, p. 7787-7793, Vol. 74, No. 17
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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