This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vink, C.
Right arrow Articles by Bruggeman, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vink, C.
Right arrow Articles by Bruggeman, C. A.

 Previous Article  |  Next Article 

Journal of Virology, August 2000, p. 7656-7665, Vol. 74, No. 16
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Complete DNA Sequence of the Rat Cytomegalovirus Genome

Cornelis Vink,* Erik Beuken, and Cathrien A. Bruggeman

Department of Medical Microbiology, Cardiovascular Research Institute Maastricht, University of Maastricht, 6202 AZ Maastricht, The Netherlands

Received 18 February 2000/Accepted 16 May 2000

We have determined the complete genome sequence of the Maastricht strain of rat cytomegalovirus (RCMV). The RCMV genome has a length of 229,896 bp and is arranged as a single unique sequence flanked by 504-bp terminal direct repeats. RCMV was found to have counterparts of all but one of the open reading frames (ORFs) that are conserved between murine CMV (MCMV) and human CMV (HCMV). Like HCMV, RCMV lacks homologs of the genes belonging to the MCMV m02 glycoprotein gene family. However, RCMV contains 15 ORFs with homology to members of the MCMV m145 glycoprotein gene family. Four ORFs are predicted to encode homologs of host proteins; R33 and R78 both putatively encode G protein-coupled receptors, whereas r144 and r131 encode homologs of major histocompatibility class I heavy chains and CC chemokines, respectively. An intriguing feature of the RCMV genome is the presence of an ORF, r127, with similarity to the rep gene of parvoviruses as well as ORF U94 of human herpesvirus 6A (HHV-6A) and HHV-6B. Counterparts of these ORFs have not been found in the other sequenced herpesviruses.

* Corresponding author. Mailing address: Department of Medical Microbiology, University of Maastricht, P.O. Box 5800, 6202 AZ Maastricht, The Netherlands. Phone: 31 43 3876669. Fax: 31 43 3876643. E-mail: kvi{at}lmib.azm.nl.

Journal of Virology, August 2000, p. 7656-7665, Vol. 74, No. 16
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Cheeran, M. C.-J., Lokensgard, J. R., Schleiss, M. R. (2009). Neuropathogenesis of Congenital Cytomegalovirus Infection: Disease Mechanisms and Prospects for Intervention. Clin. Microbiol. Rev. 22: 99-126 [Abstract] [Full Text]  
  • Case, R., Sharp, E., Benned-Jensen, T., Rosenkilde, M. M., Davis-Poynter, N., Farrell, H. E. (2008). Functional Analysis of the Murine Cytomegalovirus Chemokine Receptor Homologue M33: Ablation of Constitutive Signaling Is Associated with an Attenuated Phenotype In Vivo. J. Virol. 82: 1884-1898 [Abstract] [Full Text]  
  • Mans, J., Natarajan, K., Balbo, A., Schuck, P., Eikel, D., Hess, S., Robinson, H., Simic, H., Jonjic, S., Tiemessen, C. T., Margulies, D. H. (2007). Cellular Expression and Crystal Structure of the Murine Cytomegalovirus Major Histocompatibility Complex Class I-like Glycoprotein, m153. J. Biol. Chem. 282: 35247-35258 [Abstract] [Full Text]  
  • Streblow, D. N., van Cleef, K. W. R., Kreklywich, C. N., Meyer, C., Smith, P., Defilippis, V., Grey, F., Fruh, K., Searles, R., Bruggeman, C., Vink, C., Nelson, J. A., Orloff, S. L. (2007). Rat Cytomegalovirus Gene Expression in Cardiac Allograft Recipients Is Tissue Specific and Does Not Parallel the Profiles Detected In Vitro. J. Virol. 81: 3816-3826 [Abstract] [Full Text]  
  • Valchanova, R. S., Picard-Maureau, M., Budt, M., Brune, W. (2006). Murine Cytomegalovirus m142 and m143 Are both Required To Block Protein Kinase R-Mediated Shutdown of Protein Synthesis.. J. Virol. 80: 10181-10190 [Abstract] [Full Text]  
  • Tang, Q., Murphy, E. A., Maul, G. G. (2006). Experimental Confirmation of Global Murine Cytomegalovirus Open Reading Frames by Transcriptional Detection and Partial Characterization of Newly Described Gene Products. J. Virol. 80: 6873-6882 [Abstract] [Full Text]  
  • Brocchieri, L., Kledal, T. N., Karlin, S., Mocarski, E. S. (2005). Predicting Coding Potential from Genome Sequence: Application to Betaherpesviruses Infecting Rats and Mice. J. Virol. 79: 7570-7596 [Abstract] [Full Text]  
  • Voigt, S., Sandford, G. R., Hayward, G. S., Burns, W. H. (2005). The English strain of rat cytomegalovirus (CMV) contains a novel captured CD200 (vOX2) gene and a spliced CC chemokine upstream from the major immediate-early region: further evidence for a separate evolutionary lineage from that of rat CMV Maastricht. J. Gen. Virol. 86: 263-274 [Abstract] [Full Text]  
  • Michel, D., Milotic, I., Wagner, M., Vaida, B., Holl, J., Ansorge, R., Mertens, T. (2005). The human cytomegalovirus UL78 gene is highly conserved among clinical isolates, but is dispensable for replication in fibroblasts and a renal artery organ-culture system. J. Gen. Virol. 86: 297-306 [Abstract] [Full Text]  
  • Kattenhorn, L. M., Mills, R., Wagner, M., Lomsadze, A., Makeev, V., Borodovsky, M., Ploegh, H. L., Kessler, B. M. (2004). Identification of Proteins Associated with Murine Cytomegalovirus Virions. J. Virol. 78: 11187-11197 [Abstract] [Full Text]  
  • McGregor, A., Liu, F., Schleiss, M. R. (2004). Molecular, Biological, and In Vivo Characterization of the Guinea Pig Cytomegalovirus (CMV) Homologs of the Human CMV Matrix Proteins pp71 (UL82) and pp65 (UL83). J. Virol. 78: 9872-9889 [Abstract] [Full Text]  
  • van Cleef, K. W. R., Scaf, W. M. A., Maes, K., Kaptein, S. J. F., Beuken, E., Beisser, P. S., Stassen, F. R. M., Grauls, G. E. L. M., Bruggeman, C. A., Vink, C. (2004). The rat cytomegalovirus homologue of parvoviral rep genes, r127, encodes a nuclear protein with single- and double-stranded DNA-binding activity that is dispensable for virus replication. J. Gen. Virol. 85: 2001-2013 [Abstract] [Full Text]  
  • Smith, L. M., Tonkin, J. N., Lawson, M. A., Shellam, G. R. (2004). Isolates of cytomegalovirus (CMV) from the black rat Rattus rattus form a distinct group of rat CMV. J. Gen. Virol. 85: 1313-1317 [Abstract] [Full Text]  
  • Tulman, E. R., Afonso, C. L., Lu, Z., Zsak, L., Kutish, G. F., Rock, D. L. (2004). The Genome of Canarypox Virus. J. Virol. 78: 353-366 [Abstract] [Full Text]  
  • Mills, R., Rozanov, M., Lomsadze, A., Tatusova, T., Borodovsky, M. (2003). Improving gene annotation of complete viral genomes. Nucleic Acids Res 31: 7041-7055 [Abstract] [Full Text]  
  • Brune, W., Nevels, M., Shenk, T. (2003). Murine Cytomegalovirus m41 Open Reading Frame Encodes a Golgi-Localized Antiapoptotic Protein. J. Virol. 77: 11633-11643 [Abstract] [Full Text]  
  • van der Strate, B. W. A., Hillebrands, J. L., Lycklama a Nijeholt, S. S., Beljaars, L., Bruggeman, C. A., van Luyn, M. J. A., Rozing, J., The, T. H., Meijer, D. K. F., Molema, G., Harmsen, M. C. (2003). Dissemination of Rat Cytomegalovirus through Infected Granulocytes and Monocytes In Vitro and In Vivo. J. Virol. 77: 11274-11278 [Abstract] [Full Text]  
  • Penfold, M. E. T., Schmidt, T. L., Dairaghi, D. J., Barry, P. A., Schall, T. J. (2003). Characterization of the Rhesus Cytomegalovirus US28 Locus. J. Virol. 77: 10404-10413 [Abstract] [Full Text]  
  • Kaptein, S. J. F., Beisser, P. S., Gruijthuijsen, Y. K., Savelkouls, K. G. M., van Cleef, K. W. R., Beuken, E., Grauls, G. E. L. M., Bruggeman, C. A., Vink, C. (2003). The rat cytomegalovirus R78 G protein-coupled receptor gene is required for production of infectious virus in the spleen. J. Gen. Virol. 84: 2517-2530 [Abstract] [Full Text]  
  • Tam, A., Zhu, J., Hai, R., Haghjoo, E., Tong, T., Zhan, X., Lu, S., Liu, F. (2003). Murine Cytomegalovirus with a Transposon Insertional Mutation at Open Reading Frame M35 Is Defective in Growth In Vivo. J. Virol. 77: 7746-7755 [Abstract] [Full Text]  
  • Hansen, S. G., Strelow, L. I., Franchi, D. C., Anders, D. G., Wong, S. W. (2003). Complete Sequence and Genomic Analysis of Rhesus Cytomegalovirus. J. Virol. 77: 6620-6636 [Abstract] [Full Text]  
  • Menard, C., Wagner, M., Ruzsics, Z., Holak, K., Brune, W., Campbell, A. E., Koszinowski, U. H. (2003). Role of Murine Cytomegalovirus US22 Gene Family Members in Replication in Macrophages. J. Virol. 77: 5557-5570 [Abstract] [Full Text]  
  • Akter, P., Cunningham, C., McSharry, B. P., Dolan, A., Addison, C., Dargan, D. J., Hassan-Walker, A. F., Emery, V. C., Griffiths, P. D., Wilkinson, G. W. G., Davison, A. J. (2003). Two novel spliced genes in human cytomegalovirus. J. Gen. Virol. 84: 1117-1122 [Abstract] [Full Text]  
  • Davison, A. J., Akter, P., Cunningham, C., Dolan, A., Addison, C., Dargan, D. J., Hassan-Walker, A. F., Emery, V. C., Griffiths, P. D., Wilkinson, G. W. G. (2003). Homology between the human cytomegalovirus RL11 gene family and human adenovirus E3 genes. J. Gen. Virol. 84: 657-663 [Abstract] [Full Text]  
  • Streblow, D. N., Kreklywich, C., Yin, Q., De La Melena, V. T., Corless, C. L., Smith, P. A., Brakebill, C., Cook, J. W., Vink, C., Bruggeman, C. A., Nelson, J. A., Orloff, S. L. (2003). Cytomegalovirus-Mediated Upregulation of Chemokine Expression Correlates with the Acceleration of Chronic Rejection in Rat Heart Transplants. J. Virol. 77: 2182-2194 [Abstract] [Full Text]  
  • Davison, A. J., Dolan, A., Akter, P., Addison, C., Dargan, D. J., Alcendor, D. J., McGeoch, D. J., Hayward, G. S. (2003). The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome. J. Gen. Virol. 84: 17-28 [Abstract] [Full Text]  
  • Smith, H. R. C., Heusel, J. W., Mehta, I. K., Kim, S., Dorner, B. G., Naidenko, O. V., Iizuka, K., Furukawa, H., Beckman, D. L., Pingel, J. T., Scalzo, A. A., Fremont, D. H., Yokoyama, W. M. (2002). Recognition of a virus-encoded ligand by a natural killer cell activation receptor. Proc. Natl. Acad. Sci. USA 99: 8826-8831 [Abstract] [Full Text]  
  • Adair, R., Douglas, E. R., Maclean, J. B., Graham, S. Y., Aitken, J. D., Jamieson, F. E., Dargan, D. J. (2002). The products of human cytomegalovirus genes UL23, UL24, UL43 and US22 are tegument components. J. Gen. Virol. 83: 1315-1324 [Abstract] [Full Text]  
  • Arase, H., Mocarski, E. S., Campbell, A. E., Hill, A. B., Lanier, L. L. (2002). Direct Recognition of Cytomegalovirus by Activating and Inhibitory NK Cell Receptors. Science 296: 1323-1326 [Abstract] [Full Text]  
  • Inkinen, K., Soots, A., Krogerus, L., Bruggeman, C., Ahonen, J., Lautenschlager, I. (2002). Cytomegalovirus increases collagen synthesis in chronic rejection in the rat. Nephrol Dial Transplant 17: 772-779 [Abstract] [Full Text]  
  • Stamminger, T., Gstaiger, M., Weinzierl, K., Lorz, K., Winkler, M., Schaffner, W. (2002). Open Reading Frame UL26 of Human Cytomegalovirus Encodes a Novel Tegument Protein That Contains a Strong Transcriptional Activation Domain. J. Virol. 76: 4836-4847 [Abstract] [Full Text]  
  • Dhepakson, P., Mori, Y., Jiang, Y. B., Huang, H. L., Akkapaiboon, P., Okuno, T., Yamanishi, K. (2002). Human herpesvirus-6 rep/U94 gene product has single-stranded DNA-binding activity. J. Gen. Virol. 83: 847-854 [Abstract] [Full Text]  
  • Liu, Y., Biegalke, B. J. (2002). The Human Cytomegalovirus UL35 Gene Encodes Two Proteins with Different Functions. J. Virol. 76: 2460-2468 [Abstract] [Full Text]  
  • Gruijthuijsen, Y. K., Casarosa, P., Kaptein, S. J. F., Broers, J. L. V., Leurs, R., Bruggeman, C. A., Smit, M. J., Vink, C. (2002). The Rat Cytomegalovirus R33-Encoded G Protein-Coupled Receptor Signals in a Constitutive Fashion. J. Virol. 76: 1328-1338 [Abstract] [Full Text]  
  • Yang, F., He, J., Lin, X., Li, Q., Pan, D., Zhang, X., Xu, X. (2001). Complete Genome Sequence of the Shrimp White Spot Bacilliform Virus. J. Virol. 75: 11811-11820 [Abstract] [Full Text]  
  • Hayajneh, W. A., Contopoulos-Ioannidis, D. G., Lesperance, M. M., Venegas, A. M., Colberg-Poley, A. M. (2001). The carboxyl terminus of the human cytomegalovirus UL37 immediate-early glycoprotein is conserved in primary strains and is important for transactivation. J. Gen. Virol. 82: 1569-1579 [Abstract] [Full Text]  
  • LaPierre, L. A., Biegalke, B. J. (2001). Identification of a Novel Transcriptional Repressor Encoded by Human Cytomegalovirus. J. Virol. 75: 6062-6069 [Abstract] [Full Text]  
  • Bahr, U., Darai, G. (2001). Analysis and Characterization of the Complete Genome of Tupaia (Tree Shrew) Herpesvirus. J. Virol. 75: 4854-4870 [Abstract] [Full Text]  
  • Oliveira, S. A., Shenk, T. E. (2001). Murine cytomegalovirus M78 protein, a G protein-coupled receptor homologue, is a constituent of the virion and facilitates accumulation of immediate-early viral mRNA. Proc. Natl. Acad. Sci. USA 98: 3237-3242 [Abstract] [Full Text]  
  • Abenes, G., Lee, M., Haghjoo, E., Tong, T., Zhan, X., Liu, F. (2001). Murine Cytomegalovirus Open Reading Frame M27 Plays an Important Role in Growth and Virulence in Mice. J. Virol. 75: 1697-1707 [Abstract] [Full Text]  
  • Voigt, S., Sandford, G. R., Ding, L., Burns, W. H. (2001). Identification and Characterization of a Spliced C-Type Lectin-Like Gene Encoded by Rat Cytomegalovirus. J. Virol. 75: 603-611 [Abstract] [Full Text]  
  • Albà, M. M., Das, R., Orengo, C. A., Kellam, P. (2001). Genomewide Function Conservation and Phylogeny in the Herpesviridae. Genome Res 11: 43-54 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»