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Journal of Virology, August 2000, p. 7642-7645, Vol. 74, No. 16
Department of Pediatrics, School of
Medicine,1 and Department of
Gastroenterology,2 University of Washington,
and Veterans Affairs Medical Center,3 Seattle,
Washington, and Department of Internal Medicine,
Southwestern Medical Center, Dallas, Texas4
Received 10 August 1999/Accepted 4 May 2000
Intestinal epithelial cells secrete a protective luminal mucus
barrier inhibiting viral gene transfer. Quiescent, polarized monolayers
of primary epithelial cells from dog gallbladder and human colon are
efficiently transduced through the apical mucus side by lentivirus
vectors, suggesting their application to intestinal gene therapy.
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Apical Gene Transfer into Quiescent Human and Canine
Polarized Intestinal Epithelial Cells by Lentivirus
Vectors

*
Corresponding author. Mailing address: Department of
Pediatrics, University of Washington School of Medicine, Box 356320, Seattle, WA 98195-6320. Phone: (206) 543-4735. Fax: (206) 543-3184. E-mail: wosborne{at}u.washington.edu.
Present address: Academic Medical Center, Department of
Experimental Hepatology, 1105 AZ Amsterdam, Netherlands.
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