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Journal of Virology, August 2000, p. 7628-7635, Vol. 74, No. 16
Department of Medical
Virology1 and Department of Internal
Medicine III,2 University of Tübingen,
D-72076 Tübingen, Germany
Received 23 March 2000/Accepted 11 May 2000
Endothelial cells (EC) are common targets of permissive infection
by human cytomegalovirus (HCMV) in vivo during acute disease. However,
studies of HCMV-EC interactions in vitro have generated discordant
results. While lytic infection of cultured venous EC has been well
established, Fish et al. (K. N. Fish, C. Soderberg Naucler,
L. K. Mills, S. Stenglein, and J. A. Nelson, J. Virol. 72:5661-5668) have reported noncytopathic persistence of the virus in
cultured aortic EC. We propose that interstrain differences in viral
host cell tropism rather than the vascular bed of origin of infected EC
might account for these discrepancies. In the present investigation we
compared the responses of EC derived from human adult iliac artery,
placental microvasculature, and umbilical vein to infection with
various HCMV strains. Regardless of the vascular bed of origin,
infection with EC-propagated HCMV strains induced 100% efficient
cytopathic change progressing to complete lysis of inoculated
monolayers. While fibroblast-propagated strains persisted at low titer
in infected arterial EC cultures, they were also cytolytic for
individual infected cells. The finding of cytopathic lytic infection of
arterial EC by HCMV implicates a mechanism of vascular injury in the
pathogenesis of HCMV infection.
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Efficient Lytic Infection of Human Arterial
Endothelial Cells by Human Cytomegalovirus Strains
*
Corresponding author. Mailing address: Abt. Med.
Virologie, Universität Tübingen, Calwerstrasse 7/6, D-72076
Tübingen, Germany. Phone: 49 7071 2987459. Fax: 49 7071 295790. E-mail: christian.sinzger{at}med.uni-tuebingen.de.
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