This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Webster-Cyriaque, J.
Right arrow Articles by Raab-Traub, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Webster-Cyriaque, J.
Right arrow Articles by Raab-Traub, N.

 Previous Article  |  Next Article 

Journal of Virology, August 2000, p. 7610-7618, Vol. 74, No. 16
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Hairy Leukoplakia: an Unusual Combination of Transforming and Permissive Epstein-Barr Virus Infections

J. Webster-Cyriaque,1,2 J. Middeldorp,3 and N. Raab-Traub1,4,*

Lineberger Comprehensive Cancer Center,1 Department of Microbiology,4 and UNC School of Dentistry, Department of Dental Ecology,2 University of North Carolina, Chapel Hill, North Carolina, and Organon Teknika, Boxtel, The Netherlands3

Received 12 October 1999/Accepted 9 May 2000

Human herpesviruses are characterized by distinct states of infection. Typically in permissive herpesvirus infection, abundant virus production results in cell lysis. In latent transforming Epstein-Barr virus (EBV) infection, viral proteins that induce cell growth are expressed. The immunodeficiency-associated hairy leukoplakia (HLP) lesion is the only pathologic manifestation of permissive EBV infection; however, within HLP, viral proteins characteristic of latent infection have also been detected. In this study, we further analyzed expression of EBV latent genes and investigated their contribution to the unique histologic phenotype of HLP. Coexpression of lytic and transforming viral proteins was detected simultaneously within individual HLP keratinocytes. LMP1 has now been shown to be uniformly expressed in the affected tissue, and it is associated and colocalizes with tumor necrosis factor receptor-associated factor (TRAF) signaling molecules. Effects induced by activated TRAF signaling that were detected in HLP included activation of NF-kappa B and c-Jun terminal kinase 1 (JNK1) and upregulated expression of epidermal growth factor receptor (EGFR), CD40, A20, and TRAFs. This study identifies a novel state of EBV infection with concurrent expression of replicative and transforming proteins. It is probable that both replicative and latent proteins contribute to HLP development and induce many of the histologic features of HLP, such as acanthosis and hyperproliferation. In contrast to other permissive herpesvirus infections, expression of EBV transforming proteins within the permissively infected HLP tissue enables epithelial cell survival and may enhance viral replication.

* Corresponding author. Mailing address: Lineberger Comprehensive Cancer Center, CB 7295, University of North Carolina, Chapel Hill, NC 27599-7295. Phone: (919) 966-1701. Fax: (919) 966-3015. E-mail: nrt{at}med.unc.edu.

Journal of Virology, August 2000, p. 7610-7618, Vol. 74, No. 16
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Peferoen, L. A. N., Lamers, F., Lodder, L. N. R., Gerritsen, W. H., Huitinga, I., Melief, J., Giovannoni, G., Meier, U., Hintzen, R. Q., Verjans, G. M. G. M., van Nierop, G. P., Vos, W., Peferoen-Baert, R. M. B., Middeldorp, J. M., van der Valk, P., Amor, S. (2009). Epstein Barr virus is not a characteristic feature in the central nervous system in established multiple sclerosis. Brain 0: awp296v1-awp296 [Full Text]  
  • Shannon-Lowe, C., Adland, E., Bell, A. I., Delecluse, H.-J., Rickinson, A. B., Rowe, M. (2009). Features Distinguishing Epstein-Barr Virus Infections of Epithelial Cells and B Cells: Viral Genome Expression, Genome Maintenance, and Genome Amplification. J. Virol. 83: 7749-7760 [Abstract] [Full Text]  
  • Song, Y.-J., Izumi, K. M., Shinners, N. P., Gewurz, B. E., Kieff, E. (2008). IRF7 activation by Epstein-Barr virus latent membrane protein 1 requires localization at activation sites and TRAF6, but not TRAF2 or TRAF3. Proc. Natl. Acad. Sci. USA 105: 18448-18453 [Abstract] [Full Text]  
  • Xing, L., Kieff, E. (2007). Epstein-Barr Virus BHRF1 Micro- and Stable RNAs during Latency III and after Induction of Replication. J. Virol. 81: 9967-9975 [Abstract] [Full Text]  
  • Walling, D. M., Ray, A. J., Nichols, J. E., Flaitz, C. M., Nichols, C. M. (2007). Epstein-Barr Virus Infection of Langerhans Cell Precursors as a Mechanism of Oral Epithelial Entry, Persistence, and Reactivation. J. Virol. 81: 7249-7268 [Abstract] [Full Text]  
  • Tugizov, S., Herrera, R., Veluppillai, P., Greenspan, J., Greenspan, D., Palefsky, J. M. (2007). Epstein-Barr Virus (EBV)-Infected Monocytes Facilitate Dissemination of EBV within the Oral Mucosal Epithelium. J. Virol. 81: 5484-5496 [Abstract] [Full Text]  
  • Webster-Cyriaque, J., Duus, K., Cooper, C., Duncan, M. (2006). Oral EBV and KSHV Infection in HIV. ADR 19: 91-95 [Abstract] [Full Text]  
  • Yuan, J., Cahir-McFarland, E., Zhao, B., Kieff, E. (2006). Virus and Cell RNAs Expressed during Epstein-Barr Virus Replication. J. Virol. 80: 2548-2565 [Abstract] [Full Text]  
  • Hahn, A. M., Huye, L. E., Ning, S., Webster-Cyriaque, J., Pagano, J. S. (2005). Interferon Regulatory Factor 7 Is Negatively Regulated by the Epstein-Barr Virus Immediate-Early Gene, BZLF-1. J. Virol. 79: 10040-10052 [Abstract] [Full Text]  
  • Guidoboni, M., Ponzoni, M., Caggiari, L., Lettini, A. A., Vago, L., De Re, V., Gloghini, A., Zancai, P., Carbone, A., Boiocchi, M., Dolcetti, R. (2005). Latent Membrane Protein 1 Deletion Mutants Accumulate in Reed-Sternberg Cells of Human Immunodeficiency Virus-Related Hodgkin's Lymphoma. J. Virol. 79: 2643-2649 [Abstract] [Full Text]  
  • Chang, Y., Lee, H.-H., Chang, S.-S., Hsu, T.-Y., Wang, P.-W., Chang, Y.-S., Takada, K., Tsai, C.-H. (2004). Induction of Epstein-Barr Virus Latent Membrane Protein 1 by a Lytic Transactivator Rta. J. Virol. 78: 13028-13036 [Abstract] [Full Text]  
  • Pegtel, D. M., Middeldorp, J., Thorley-Lawson, D. A. (2004). Epstein-Barr Virus Infection in Ex Vivo Tonsil Epithelial Cell Cultures of Asymptomatic Carriers. J. Virol. 78: 12613-12624 [Abstract] [Full Text]  
  • Everly, D. N. Jr., Kusano, S., Raab-Traub, N. (2004). Accumulation of Cytoplasmic {beta}-Catenin and Nuclear Glycogen Synthase Kinase 3{beta} in Epstein-Barr Virus-Infected Cells. J. Virol. 78: 11648-11655 [Abstract] [Full Text]  
  • Li, Y., Mahajan, N. P., Webster-Cyriaque, J., Bhende, P., Hong, G. K., Earp, H. S., Kenney, S. (2004). The C-Mer Gene Is Induced by Epstein-Barr Virus Immediate-Early Protein BRLF1. J. Virol. 78: 11778-11785 [Abstract] [Full Text]  
  • Duus, K. M., Lentchitsky, V., Wagenaar, T., Grose, C., Webster-Cyriaque, J. (2004). Wild-Type Kaposi's Sarcoma-Associated Herpesvirus Isolated from the Oropharynx of Immune-Competent Individuals Has Tropism for Cultured Oral Epithelial Cells. J. Virol. 78: 4074-4084 [Abstract] [Full Text]  
  • Li, Y., Webster-Cyriaque, J., Tomlinson, C. C., Yohe, M., Kenney, S. (2004). Fatty Acid Synthase Expression Is Induced by the Epstein-Barr Virus Immediate-Early Protein BRLF1 and Is Required for Lytic Viral Gene Expression. J. Virol. 78: 4197-4206 [Abstract] [Full Text]  
  • Huang, J., Chen, H., Hutt-Fletcher, L., Ambinder, R. F., Hayward, S. D. (2003). Lytic Viral Replication as a Contributor to the Detection of Epstein-Barr Virus in Breast Cancer. J. Virol. 77: 13267-13274 [Abstract] [Full Text]  
  • Prince, S., Keating, S., Fielding, C., Brennan, P., Floettmann, E., Rowe, M. (2003). Latent Membrane Protein 1 Inhibits Epstein-Barr Virus Lytic Cycle Induction and Progress via Different Mechanisms. J. Virol. 77: 5000-5007 [Abstract] [Full Text]  
  • Kraus, R. J., Perrigoue, J. G., Mertz, J. E. (2002). ZEB Negatively Regulates the Lytic-Switch BZLF1 Gene Promoter of Epstein-Barr Virus. J. Virol. 77: 199-207 [Abstract] [Full Text]  
  • Mauser, A., Holley-Guthrie, E., Zanation, A., Yarborough, W., Kaufmann, W., Klingelhutz, A., Seaman, W. T., Kenney, S. (2002). The Epstein-Barr Virus Immediate-Early Protein BZLF1 Induces Expression of E2F-1 and Other Proteins Involved in Cell Cycle Progression in Primary Keratinocytes and Gastric Carcinoma Cells. J. Virol. 76: 12543-12552 [Abstract] [Full Text]  
  • Mauser, A., Holley-Guthrie, E., Simpson, D., Kaufmann, W., Kenney, S. (2002). The Epstein-Barr Virus Immediate-Early Protein BZLF1 Induces both a G2 and a Mitotic Block. J. Virol. 76: 10030-10037 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»