This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Weidmann, A.
Right arrow Articles by Schneider-Schaulies, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Weidmann, A.
Right arrow Articles by Schneider-Schaulies, S.

 Previous Article  |  Next Article 

Journal of Virology, August 2000, p. 7548-7553, Vol. 74, No. 16
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Measles Virus-Induced Immunosuppression In Vitro Is Independent of Complex Glycosylation of Viral Glycoproteins and of Hemifusion

Armin Weidmann,1 Christian Fischer,2 Shinji Ohgimoto,1 Claudia Rüth,1 Volker ter Meulen,1,* and Sibylle Schneider-Schaulies1

Institute for Virology and Immunobiology, University of Würzburg, D-97078 Würzburg,1 and Institute for Virology, University of Marburg, D-35037 Marburg,2 Germany

Received 29 November 1999/Accepted 18 May 2000

Expression of the measles virus (MV) F/H complex on the surface of viral particles, infected cells, or cells transfected to express these proteins (presenter cells [PC]) is necessary and sufficient to induce proliferative arrest in both human and rodent lymphoid cells (responder cells [RC]). This inhibition was found to occur independent of apoptosis and soluble mediators excluded by a pore size filter of 200 nm released from either PC or RC. We now show that reactive oxygen intermediates which might be released by RC or PC also do not contribute to MV-induced immunosuppression in vitro. Using an inhibitor of Golgi-resident mannosidases (deoxymannojirimycin), we found that complex glycosylation of the F and H proteins is not required for the induction of proliferative arrest of RC. As revealed by our previous studies, proteolytic cleavage of the MV F protein precursor into its F1 and F2 subunits, but not of F/H-mediated cellular fusion, was found to be required, since fusion-inhibitory peptides such as Z-D-Phe-L-Phe-Gly (Z-fFG) did not interfere with the induction of proliferative inhibition. We now show that Z-fFG inhibits cellular fusion at the stage of hemifusion by preventing lipid mixing of the outer membrane layer. These results provide strong evidence for a receptor-mediated signal elicited by the MV F/H complex which can be uncoupled from its fusogenic activity is required for the induction of proliferative arrest of human lymphocytes.

* Corresponding author. Mailing address: Institute for Virology, Versbacher Str. 7, D-97078 Würzburg, Germany. Phone: 49-931-201-3895. Fax: 49-931-201-5954. E-mail: termeulen{at}vim.uni-wuerzburg.de.

Journal of Virology, August 2000, p. 7548-7553, Vol. 74, No. 16
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Runkler, N., Dietzel, E., Carsillo, M., Niewiesk, S., Maisner, A. (2009). Sorting signals in the measles virus wild-type glycoproteins differently influence virus spread in polarized epithelia and lymphocytes. J. Gen. Virol. 90: 2474-2482 [Abstract] [Full Text]  
  • Jerome, K. R. (2008). Viral Modulation of T-Cell Receptor Signaling. J. Virol. 82: 4194-4204 [Full Text]  
  • Schneider-Schaulies, S., Dittmer, U. (2006). Silencing T cells or T-cell silencing: concepts in virus-induced immunosuppression. J. Gen. Virol. 87: 1423-1438 [Abstract] [Full Text]  
  • Song, M. K., Vindurampulle, C. J., Capozzo, A. V. E., Ulmer, J., Polo, J. M., Pasetti, M. F., Barry, E. M., Levine, M. M. (2005). Characterization of Immune Responses Induced by Intramuscular Vaccination with DNA Vaccines Encoding Measles Virus Hemagglutinin and/or Fusion Proteins. J. Virol. 79: 9854-9861 [Abstract] [Full Text]  
  • Klagge, I. M., Abt, M., Fries, B., Schneider-Schaulies, S. (2004). Impact of measles virus dendritic-cell infection on Th-cell polarization in vitro. J. Gen. Virol. 85: 3239-3247 [Abstract] [Full Text]  
  • Avota, E., Muller, N., Klett, M., Schneider-Schaulies, S. (2004). Measles Virus Interacts with and Alters Signal Transduction in T-Cell Lipid Rafts. J. Virol. 78: 9552-9559 [Abstract] [Full Text]  
  • Bieback, K., Lien, E., Klagge, I. M., Avota, E., Schneider-Schaulies, J., Duprex, W. P., Wagner, H., Kirschning, C. J., ter Meulen, V., Schneider-Schaulies, S. (2002). Hemagglutinin Protein of Wild-Type Measles Virus Activates Toll-Like Receptor 2 Signaling. J. Virol. 76: 8729-8736 [Abstract] [Full Text]  
  • Moll, M., Klenk, H.-D., Maisner, A. (2002). Importance of the Cytoplasmic Tails of the Measles Virus Glycoproteins for Fusogenic Activity and the Generation of Recombinant Measles Viruses. J. Virol. 76: 7174-7186 [Abstract] [Full Text]  
  • Schlender, J., Walliser, G., Fricke, J., Conzelmann, K.-K. (2002). Respiratory Syncytial Virus Fusion Protein Mediates Inhibition of Mitogen-Induced T-Cell Proliferation by Contact. J. Virol. 76: 1163-1170 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»