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Journal of Virology, August 2000, p. 7381-7390, Vol. 74, No. 16
A. N. Belozersky Institute of
Physical-Chemical Biology, Moscow State University, Moscow
119899,1 and M. P. Chumakov
Institute of Poliomyelitis and Viral Encephalitides, Russian Academy
of Medical Sciences, Moscow Region 142782,3
Russia, and National Center for Infectious Diseases,
Centers for Disease Control and Prevention, Atlanta, Georgia
303332
Received 15 March 2000/Accepted 18 May 2000
We determined nucleotide sequences of the VP1 and 2AB genes and
portions of the 2C and 3D genes of two evolving poliovirus lineages:
circulating wild viruses of T geotype and Sabin vaccine-derived isolates from an immunodeficient patient. Different regions of the
viral RNA were found to evolve nonsynchronously, and the rate of
evolution of the 2AB region in the vaccine-derived population was not
constant throughout its history. Synonymous replacements occurred not
completely randomly, suggesting the need for conservation of certain
rare codons (possibly to control translation elongation) and the
existence of unidentified constraints in the viral RNA structure.
Nevertheless the major contribution to the evolution of the two
lineages came from linear accumulation of synonymous substitutions.
Therefore, in agreement with current theories of viral evolution, we
suggest that the majority of the mutations in both lineages were fixed
as a result of successive sampling, from the heterogeneous populations,
of random portions containing predominantly neutral and possibly
adverse mutations. As a result of such a mode of evolution, the virus
fitness may be maintained at a more or less constant level or may
decrease unless more-fit variants are stochastically generated. The
proposed unifying model of natural poliovirus evolution has important
implications for the epidemiology of poliomyelitis.
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Evolution of Circulating Wild Poliovirus and of Vaccine-Derived
Poliovirus in an Immunodeficient Patient: a Unifying Model
*
Corresponding author. Mailing address: Institute of
Poliomyelitis, Moscow Region 142782, Russia. Phone: 7 (095) 439 9026. Fax: 7 (095) 439 9321. E-mail:
viago{at}ipive.genebee.msu.su.
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