This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Endo, Y.
Right arrow Articles by Martin, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Endo, Y.
Right arrow Articles by Martin, M. A.

 Previous Article  |  Next Article 

Journal of Virology, August 2000, p. 6935-6945, Vol. 74, No. 15
0022-538X/00/$04.00+0

Short- and Long-Term Clinical Outcomes in Rhesus Monkeys Inoculated with a Highly Pathogenic Chimeric Simian/Human Immunodeficiency Virus

Yasuyuki Endo,1 Tatsuhiko Igarashi,1 Yoshiaki Nishimura,1 Charles Buckler,1 Alicia Buckler-White,1 Ronald Plishka,1 Dimiter S. Dimitrov,2 and Malcolm A. Martin1,*

Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0460,1 and Laboratory of Experimental and Computational Biology, National Cancer Institute, National Institutes of Health, Frederick, Maryland 217022

Received 29 February 2000/Accepted 9 May 2000

A highly pathogenic simian/human immunodeficiency virus (SHIV), SHIVDH12R, isolated from a rhesus macaque that had been treated with anti-human CD8 monoclonal antibody at the time of primary infection with the nonpathogenic, molecularly cloned SHIVDH12, induced marked and rapid CD4+ T cell loss in all rhesus macaques intravenously inoculated with 1.0 50% tissue culture infective dose (TCID50) to 4.1 × 105 TCID50s of virus. Animals inoculated with 650 TCID50s of SHIVDH12R or more experienced irreversible CD4+ T lymphocyte depletion and developed clinical disease requiring euthanasia between weeks 12 and 23 postinfection. In contrast, the CD4+ T-cell numbers in four of five monkeys receiving 25 TCID50s of SHIVDH12R or less stabilized at low levels, and these surviving animals produced antibodies capable of neutralizing SHIVDH12R. In the fifth monkey, no recovery from the CD4+ T cell decline occurred, and the animal had to be euthanized. Viral RNA levels, subsequent to the initial peak of infection but not at peak viremia, correlated with the virus inoculum size and the eventual clinical course. Both initial infection rate constants, k, and decay constants, d, were determined, but only the latter were statistically correlated to clinical outcome. The attenuating effects of reduced inoculum size were also observed when virus was inoculated by the mucosal route. Because the uncloned SHIVDH12R stock possessed the genetic properties of a lentivirus quasispecies, we were able to assess the evolution of the input virus swarm in animals surviving the acute infection by monitoring the emergence of neutralization escape viral variants.

* Corresponding author. Mailing address: Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, NIH, Bldg. 4, Rm. 315, 4 Ctr. Dr., MSC 0460, Bethesda, MD 20892-0460. Phone: (301) 496-4012. Fax: (301) 402-0226. E-mail: mmartin{at}niaid.nih.gov.

Journal of Virology, August 2000, p. 6935-6945, Vol. 74, No. 15
0022-538X/00/$04.00+0


This article has been cited by other articles:

  • Kubo, M., Nishimura, Y., Shingai, M., Lee, W., Brenchley, J., Lafont, B., Buckler-White, A., Igarashi, T., Martin, M. A. (2009). Initiation of Antiretroviral Therapy 48 Hours after Infection with Simian Immunodeficiency Virus Potently Suppresses Acute-Phase Viremia and Blocks the Massive Loss of Memory CD4+ T Cells but Fails To Prevent Disease. J. Virol. 83: 7099-7108 [Abstract] [Full Text]  
  • Kader, M., Hassan, W. M., Eberly, M., Piatak, M., Lifson, J. D., Roederer, M., Mattapallil, J. J. (2008). Antiretroviral Therapy prior to Acute Viral Replication Preserves CD4 T Cells in the Periphery but Not in Rectal Mucosa during Acute Simian Immunodeficiency Virus Infection. J. Virol. 82: 11467-11471 [Abstract] [Full Text]  
  • Igarashi, T., Iyengar, R., Byrum, R. A., Buckler-White, A., Dewar, R. L., Buckler, C. E., Lane, H. C., Kamada, K., Adachi, A., Martin, M. A. (2007). Human Immunodeficiency Virus Type 1 Derivative with 7% Simian Immunodeficiency Virus Genetic Content Is Able To Establish Infections in Pig-Tailed Macaques. J. Virol. 81: 11549-11552 [Abstract] [Full Text]  
  • Igarashi, T., Donau, O. K., Imamichi, H., Nishimura, Y., Theodore, T. S., Iyengar, R., Erb, C., Buckler-White, A., Buckler, C. E., Martin, M. A. (2007). Although Macrophage-Tropic Simian/Human Immunodeficiency Viruses Can Exhibit a Range of Pathogenic Phenotypes, a Majority of Isolates Induce No Clinical Disease in Immunocompetent Macaques. J. Virol. 81: 10669-10679 [Abstract] [Full Text]  
  • Pereira, A. (2007). Cryoprecipitate versus commercial fibrinogen concentrate in patients who occasionally require a therapeutic supply of fibrinogen: risk comparison in the case of an emerging transfusion-transmitted infection. haematol 92: 846-849 [Abstract] [Full Text]  
  • Nishimura, Y., Igarashi, T., Buckler-White, A., Buckler, C., Imamichi, H., Goeken, R. M., Lee, W. R., Lafont, B. A. P., Byrum, R., Lane, H. C., Hirsch, V. M., Martin, M. A. (2007). Loss of Naive Cells Accompanies Memory CD4+ T-Cell Depletion during Long-Term Progression to AIDS in Simian Immunodeficiency Virus-Infected Macaques. J. Virol. 81: 893-902 [Abstract] [Full Text]  
  • Mattapallil, J. J., Douek, D. C., Buckler-White, A., Montefiori, D., Letvin, N. L., Nabel, G. J., Roederer, M. (2006). Vaccination preserves CD4 memory T cells during acute simian immunodeficiency virus challenge. JEM 203: 1533-1541 [Abstract] [Full Text]  
  • Mao, H., Lafont, B. A. P., Igarashi, T., Nishimura, Y., Brown, C., Hirsch, V., Buckler-White, A., Sadjadpour, R., Martin, M. A. (2005). CD8+ and CD20+ Lymphocytes Cooperate To Control Acute Simian Immunodeficiency Virus/Human Immunodeficiency Virus Chimeric Virus Infections in Rhesus Monkeys: Modulation by Major Histocompatibility Complex Genotype. J. Virol. 79: 14887-14898 [Abstract] [Full Text]  
  • Nishimura, Y., Brown, C. R., Mattapallil, J. J., Igarashi, T., Buckler-White, A., Lafont, B. A. P., Hirsch, V. M., Roederer, M., Martin, M. A. (2005). Resting naive CD4+ T cells are massively infected and eliminated by X4-tropic simian-human immunodeficiency viruses in macaques. Proc. Natl. Acad. Sci. USA 102: 8000-8005 [Abstract] [Full Text]  
  • Nishimura, Y., Igarashi, T., Donau, O. K., Buckler-White, A., Buckler, C., Lafont, B. A. P., Goeken, R. M., Goldstein, S., Hirsch, V. M., Martin, M. A. (2004). Highly pathogenic SHIVs and SIVs target different CD4+ T cell subsets in rhesus monkeys, explaining their divergent clinical courses. Proc. Natl. Acad. Sci. USA 101: 12324-12329 [Abstract] [Full Text]  
  • Sadjadpour, R., Theodore, T. S., Igarashi, T., Donau, O. K., Plishka, R. J., Buckler-White, A., Martin, M. A. (2004). Induction of Disease by a Molecularly Cloned Highly Pathogenic Simian Immunodeficiency Virus/Human Immunodeficiency Virus Chimera Is Multigenic. J. Virol. 78: 5513-5519 [Abstract] [Full Text]  
  • Igarashi, T., Donau, O. K., Imamichi, H., Dumaurier, M.-J., Sadjadpour, R., Plishka, R. J., Buckler-White, A., Buckler, C., Suffredini, A. F., Lane, H. C., Moore, J. P., Martin, M. A. (2003). Macrophage-Tropic Simian/Human Immunodeficiency Virus Chimeras Use CXCR4, Not CCR5, for Infections of Rhesus Macaque Peripheral Blood Mononuclear Cells and Alveolar Macrophages. J. Virol. 77: 13042-13052 [Abstract] [Full Text]  
  • Nishimura, Y., Igarashi, T., Haigwood, N. L., Sadjadpour, R., Donau, O. K., Buckler, C., Plishka, R. J., Buckler-White, A., Martin, M. A. (2003). Transfer of neutralizing IgG to macaques 6 h but not 24 h after SHIV infection confers sterilizing protection: Implications for HIV-1 vaccine development. Proc. Natl. Acad. Sci. USA 100: 15131-15136 [Abstract] [Full Text]  
  • Igarashi, T., Imamichi, H., Brown, C. R., Hirsch, V. M., Martin, M. A. (2003). The emergence and characterization of macrophage-tropic SIV/HIV chimeric viruses (SHIVs) present in CD4+ T cell-depleted rhesus monkeys. J. Leukoc. Biol. 74: 772-780 [Abstract] [Full Text]  
  • Igarashi, T., Endo, Y., Nishimura, Y., Buckler, C., Sadjadpour, R., Donau, O. K., Dumaurier, M.-J., Plishka, R. J., Buckler-White, A., Martin, M. A. (2003). Early Control of Highly Pathogenic Simian Immunodeficiency Virus/Human Immunodeficiency Virus Chimeric Virus Infections in Rhesus Monkeys Usually Results in Long-Lasting Asymptomatic Clinical Outcomes. J. Virol. 77: 10829-10840 [Abstract] [Full Text]  
  • Willey, R. L., Byrum, R., Piatak, M., Kim, Y. B., Cho, M. W., Rossio, J. L. Jr., Bess, J. Jr., Igarashi, T., Endo, Y., Arthur, L. O., Lifson, J. D., Martin, M. A. (2002). Control of Viremia and Prevention of Simian-Human Immunodeficiency Virus-Induced Disease in Rhesus Macaques Immunized with Recombinant Vaccinia Viruses plus Inactivated Simian Immunodeficiency Virus and Human Immunodeficiency Virus Type 1 Particles. J. Virol. 77: 1163-1174 [Abstract] [Full Text]  
  • Imamichi, H., Igarashi, T., Imamichi, T., Donau, O. K., Endo, Y., Nishimura, Y., Willey, R. L., Suffredini, A. F., Lane, H. C., Martin, M. A. (2002). Amino acid deletions are introduced into the V2 region of gp120 during independent pathogenic simian immunodeficiency virus/HIV chimeric virus (SHIV) infections of rhesus monkeys generating variants that are macrophage tropic. Proc. Natl. Acad. Sci. USA 99: 13813-13818 [Abstract] [Full Text]  
  • Nishimura, Y., Igarashi, T., Haigwood, N., Sadjadpour, R., Plishka, R. J., Buckler-White, A., Shibata, R., Martin, M. A. (2002). Determination of a Statistically Valid Neutralization Titer in Plasma That Confers Protection against Simian-Human Immunodeficiency Virus Challenge following Passive Transfer of High-Titered Neutralizing Antibodies. J. Virol. 76: 2123-2130 [Abstract] [Full Text]  
  • Chakrabarti, L. A., Ivanovic, T., Cheng-Mayer, C. (2002). Properties of the Surface Envelope Glycoprotein Associated with Virulence of Simian-Human Immunodeficiency Virus SHIVSF33A Molecular Clones. J. Virol. 76: 1588-1599 [Abstract] [Full Text]  
  • Igarashi, T., Brown, C. R., Byrum, R. A., Nishimura, Y., Endo, Y., Plishka, R. J., Buckler, C., Buckler-White, A., Miller, G., Hirsch, V. M., Martin, M. A. (2002). Rapid and Irreversible CD4+ T-Cell Depletion Induced by the Highly Pathogenic Simian/Human Immunodeficiency Virus SHIVDH12R Is Systemic and Synchronous. J. Virol. 76: 379-391 [Abstract] [Full Text]  
  • Igarashi, T., Brown, C. R., Endo, Y., Buckler-White, A., Plishka, R., Bischofberger, N., Hirsch, V., Martin, M. A. (2000). Macrophage are the principal reservoir and sustain high virus loads in rhesus macaques after the depletion of CD4+ T cells by a highly pathogenic simian immunodeficiency virus/HIV type 1 chimera (SHIV): Implications for HIV-1 infections of humans. Proc. Natl. Acad. Sci. USA 10.1073/pnas.021551798v1 [Abstract] [Full Text]  
  • Igarashi, T., Brown, C. R., Endo, Y., Buckler-White, A., Plishka, R., Bischofberger, N., Hirsch, V., Martin, M. A. (2001). Macrophage are the principal reservoir and sustain high virus loads in rhesus macaques after the depletion of CD4+ T cells by a highly pathogenic simian immunodeficiency virus/HIV type 1 chimera (SHIV): Implications for HIV-1 infections of humans. Proc. Natl. Acad. Sci. USA 98: 658-663 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»