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Journal of Virology, August 2000, p. 6856-6865, Vol. 74, No. 15
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Sequences of Citrus Tristeza Virus Separated in Time and Space Are Essentially Identical†

María R. Albiach-Martí,1 Munir Mawassi,1 Siddarame Gowda,1 Tatineni Satyanarayana,1 Mark E. Hilf,2 Savita Shanker,3 Ernesto C. Almira,3 María C. Vives,4 Carmelo López,5 Jose Guerri,4 Ricardo Flores,5 Pedro Moreno,4 Steve M. Garnsey,1,2 and William O. Dawson1,*

Citrus Research and Education Center, Department of Plant Pathology, University of Florida, Lake Alfred, Florida 338501; USDA-ARS Horticultural Research Laboratory, Orlando, Florida 328032; Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, Florida 326113; and Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada,4 and Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica-Consejo Superior de Investigaciones Científicas,5 Valencia, Spain

Received 4 January 2000/Accepted 29 April 2000

The first Citrus tristeza virus (CTV) genomes completely sequenced (19.3-kb positive-sense RNA), from four biologically distinct isolates, are unexpectedly divergent in nucleotide sequence (up to 60% divergence). Understanding of whether these large sequence differences resulted from recent evolution is important for the design of disease management strategies, particularly the use of genetically engineered mild (essentially symptomless)-strain cross protection and RNA-mediated transgenic resistance. The complete sequence of a mild isolate (T30) which has been endemic in Florida for about a century was found to be nearly identical to the genomic sequence of a mild isolate (T385) from Spain. Moreover, samples of sequences of other isolates from distinct geographic locations, maintained in different citrus hosts and also separated in time (B252 from Taiwan, B272 from Colombia, and B354 from California), were nearly identical to the T30 sequence. The sequence differences between these isolates were within or near the range of variability of the T30 population. A possible explanation for these results is that the parents of isolates T30, T385, B252, B272, and B354 have a common origin, probably Asia, and have changed little since they were dispersed throughout the world by the movement of citrus. Considering that the nucleotide divergence among the other known CTV genomes is much greater than those expected for strains of the same virus, the remarkable similarity of these five isolates indicates a high degree of evolutionary stasis in some CTV populations.

* Corresponding author. Mailing address: University of Florida, Department of Plant Pathology, Citrus Research and Education Center, 700 Experiment Station Rd., Lake Alfred, FL 33850. Phone: (863) 956-1151. Fax: (863) 956-4631. E-mail: wodtmv{at}lal.ufl.edu.

dagger University of Florida Agricultural Experiment Station journal series no. R-G7529.

Journal of Virology, August 2000, p. 6856-6865, Vol. 74, No. 15
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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