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Journal of Virology, August 2000, p. 6748-6759, Vol. 74, No. 15
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Type 2 Cytokines Predominate in the Human CD4+ T-Lymphocyte Response to Epstein-Barr Virus Nuclear Antigen 1

Patricia Steigerwald-Mullen,1,2 Michael G. Kurilla,1,2,3 and Thomas J. Braciale1,2,3,*

Beirne B. Carter Center for Immunology Research1 and Departments of Pathology2 and Microbiology,3 University of Virginia Health Sciences Center, Charlottesville, Virginia 22908

Received 15 December 1999/Accepted 3 May 2000

Epstein-Barr virus (EBV) is a ubiquitous human herpesvirus that persistently infects 85% of the adult population worldwide. In this report, we examine the proliferative response and cytokine secretion profile of CD4+ T lymphocytes from a panel of unrelated EBV-positive donors against two EBV latent antigens, EBNA1 and EBNA3C. Substantial proliferative responses by CD4+ lymphocytes were demonstrated to both antigens in multiple, randomly selected donors. Surprisingly, we observed a striking and consistent difference in cytokine response to EBNA1 and EBNA3C. EBNA1-specific CD4+ T lymphocytes from multiple unrelated donors preferentially produced type 2-like cytokines in response to antigenic stimulation, while the response to EBNA3C was a characteristic type 1 response. The implications of these findings for EBV persistence and the development of EBV-associated malignancies are discussed.

* Corresponding author. Mailing address: Beirne Carter Center for Immunology Research, P.O. Box 801386, 400 Lane Road, MR-4 Building, Rm. 4012, University of Virginia, Charlottesville, VA 22908. Phone: (804) 924-1219. Fax: (804) 924-1221. E-mail: tjb2r{at}virginia.edu.

Journal of Virology, August 2000, p. 6748-6759, Vol. 74, No. 15
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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