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Journal of Virology, July 2000, p. 6564-6569, Vol. 74, No. 14
DNAVEC Research Inc., Tsukuba-shi, Ibaraki
305-0856,1 and Department of Viral
Infection, Institute of Medical Sciences, University of Tokyo,
Minato-ku, Tokyo 108-8639,2 Japan
Received 18 January 2000/Accepted 6 April 2000
We have recovered a virion from defective cDNA of Sendai
virus (SeV) that is capable of self-replication but incapable of transmissible-virion production. This virion delivers and expresses foreign genes in infected cells, and this is the first report of a gene
expression vector derived from a defective viral genome of the
Paramyxoviridae. First, functional ribonucleoprotein
complexes (RNPs) were recovered from SeV cloned cDNA defective in the F (envelope fusion protein) gene, in the presence of plasmids expressing nucleocapsid protein and viral RNA polymerase. Then the RNPs were transfected to the cells inducibly expressing F protein. Virion-like particles thus obtained had a titer of 0.5 × 108 to
1.0 × 108 cell infectious units/ml and contained
F-defective RNA genome. This defective vector amplified specifically in
an F-expressing packaging cell line in a trypsin-dependent manner but
did not spread to F-nonexpressing cells. This vector infected and
expressed an enhanced green fluorescent protein reporter gene in
various types of animal and human cells, including nondividing cells, with high efficiency. These results suggest that this vector has great
potential for use in human gene therapy and vaccine delivery systems.
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
A Cytoplasmic RNA Vector Derived from
Nontransmissible Sendai Virus with Efficient Gene Transfer and
Expression

and
*
Corresponding author. Mailing address: DNAVEC Research
Inc., 1-25-11 Kannondai, Tsukuba-shi, Ibaraki 305-0856, Japan. Phone: 81-298-38-0540. Fax: 81-298-39-1123. E-mail:
iida{at}dnavec.co.jp.
Present address: Department of Viral Diseases and Vaccine Control,
National Institute of Infectious Diseases, Tokyo 208-0011, Japan.
Present address: AIDS Research Center, National Institute of
Infectious Diseases, Tokyo 162-8640, Japan.
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