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Journal of Virology, July 2000, p. 6485-6493, Vol. 74, No. 14
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Characterization of a Region of the Measles Virus Hemagglutinin Sufficient for Its Dimerization

Richard K. Plemper,* Anthea L. Hammond, and Roberto Cattaneo

Molecular Medicine Program, Mayo Foundation, Rochester, Minnesota 55905

Received 8 February 2000/Accepted 18 April 2000

Attachment of measles virus (MV) to its cellular receptor is mediated by the viral envelope glycoprotein hemagglutinin (H). H exists at the viral surface as a disulfide-linked dimer which may associate into a tetramer. We aimed to define regions of H essential for its homo-oligomerization. To delineate these more precisely, we have generated a series of H ectodomain truncation mutants and studied their abilities to form both homotypic complexes and heterotypic complexes with full-length H. We define a "minimal unit" which is sufficient for MV H dimerization as that encompassing residues 1 to 151. This unit forms both homodimers and heterodimers with full-length H protein, although neither is transported to the cell surface even in the presence of other MV proteins. We show that cysteine residues at positions 139 and 154 are both critical in mediating covalent dimerization, not only of the truncated H mutants but also of full-length MV H protein. Even those cysteine mutants unable to form covalent intermolecular interactions are biologically active, mediating the formation of syncytia, albeit at a reduced rate. We demonstrate that this impaired capacity to mediate cell-to-cell fusion is based mainly on a reduced transport rate of the mutant molecules to the cell surface, indicating a role for covalent intermolecular interactions in efficient transport of MV H dimers to the cell surface.

* Corresponding author. Mailing address: Molecular Medicine Program, Guggenheim 18, Mayo Foundation, 200 First St., S.W., Rochester, MN 55905. Phone: (507) 538-1105. Fax: (507) 266-4797. E-mail: plemper.richard{at}mayo.edu.

Journal of Virology, July 2000, p. 6485-6493, Vol. 74, No. 14
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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