This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pearson, M. N.
Right arrow Articles by Rohrmann, G. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pearson, M. N.
Right arrow Articles by Rohrmann, G. F.

 Previous Article  |  Next Article 

Journal of Virology, July 2000, p. 6126-6131, Vol. 74, No. 13
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification of the Lymantria dispar Nucleopolyhedrovirus Envelope Fusion Protein Provides Evidence for a Phylogenetic Division of the Baculoviridaedagger

Margot N. Pearson, Christoph Groten, and George F. Rohrmann*

Department of Microbiology, Oregon State University, Corvallis, Oregon 97331-3804

Received 18 January 2000/Accepted 3 April 2000

The complete genome sequences of a number of diverse members of the Baculoviridae including both nucleopolyhedroviruses (NPVs) and granuloviruses (GVs) revealed that they lack a homolog of GP64, the envelope fusion protein of the budded form of Autographa californica multinucleocapsid NPV (AcMNPV) and its close relatives. Computer-assisted analyses of the genome of one of these viruses, Lymantria dispar MNPV (LdMNPV), revealed a single open reading frame (ld130) whose product had the predicted properties of a membrane protein. Characterization of the localization of the products of the full-length ld130 gene and of an ld130-enhanced green fluorescent protein gene (egfp) fusion using both immunofluorescence and fluorescence microscopy revealed that LD130 accumulates at the plasma membranes of cells infected with LdMNPV or transfected with ld130-egfp. In addition, cells transfected with either ld130 or ld130-egfp or infected with wild-type virus undergo membrane fusion at pH 5. Western blot analyses indicate that LD130 is present in infected cells as an 83-kDa protein and is also present in budded virions as a protein doublet containing bands of 81 and 83 kDa. Tunicamycin treatment of infected cells resulted in an immunoreactive band of about 72 kDa, indicating that LD130 is N-glycosylated. Whereas the distribution of gp64 appears to be confined to a relatively closely related group of NPVs, homologs of ld130 are present in a diverse number of both NPVs and GVs. This suggests that LD130 may be the primordial baculovirus envelope fusion protein.

* Corresponding author. Mailing address: Department of Microbiology, Nash Hall 220, Oregon State University, Corvallis, OR 97331-7301. Phone: (541) 737-1793. Fax: (541) 737-0496. E-mail: rohrmann{at}bcc.orst.edu.

dagger Technical report 11650 from the Oregon State University Agricultural Experiment Station.

Journal of Virology, July 2000, p. 6126-6131, Vol. 74, No. 13
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Yu, I.-L., Bray, D., Lin, Y.-C., Lung, O. (2009). Autographa californica multiple nucleopolyhedrovirus ORF 23 null mutant produces occlusion-derived virions with fewer nucleocapsids. J. Gen. Virol. 90: 1499-1504 [Abstract] [Full Text]  
  • Hilton, S., Winstanley, D. (2008). Genomic sequence and biological characterization of a nucleopolyhedrovirus isolated from the summer fruit tortrix, Adoxophyes orana. J. Gen. Virol. 89: 2898-2908 [Abstract] [Full Text]  
  • Slack, J. M., Lawrence, S. D., Krell, P. J., Arif, B. M. (2008). Trypsin cleavage of the baculovirus occlusion-derived virus attachment protein P74 is prerequisite in per os infection. J. Gen. Virol. 89: 2388-2397 [Abstract] [Full Text]  
  • Wang, M., Tan, Y., Yin, F., Deng, F., Vlak, J. M., Hu, Z., Wang, H. (2008). The F-Like Protein Ac23 Enhances the Infectivity of the Budded Virus of gp64-Null Autographa californica Multinucleocapsid Nucleopolyhedrovirus Pseudotyped with Baculovirus Envelope Fusion Protein F. J. Virol. 82: 9800-9804 [Abstract] [Full Text]  
  • Tan, Y., Jiang, L., Wang, M., Yin, F., Deng, F., Liu, M., Hu, Z., Wang, H. (2008). Mutagenesis and Nuclear Magnetic Resonance Analyses of the Fusion Peptide of Helicoverpa armigera Single Nucleocapsid Nucleopolyhedrovirus F Protein. J. Virol. 82: 8138-8148 [Abstract] [Full Text]  
  • Zhou, J., Blissard, G. W. (2008). Identification of a GP64 Subdomain Involved in Receptor Binding by Budded Virions of the Baculovirus Autographica californica Multicapsid Nucleopolyhedrovirus. J. Virol. 82: 4449-4460 [Abstract] [Full Text]  
  • Li, Z., Blissard, G. W. (2008). Functional Analysis of the Transmembrane (TM) Domain of the Autographa californica Multicapsid Nucleopolyhedrovirus GP64 Protein: Substitution of Heterologous TM Domains. J. Virol. 82: 3329-3341 [Abstract] [Full Text]  
  • Wang, M., Tan, Y., Yin, F., Deng, F., Vlak, J. M., Hu, Z., Wang, H. (2008). The F protein of Helicoverpa armigera single nucleopolyhedrovirus can be substituted functionally with its homologue from Spodoptera exigua multiple nucleopolyhedrovirus. J. Gen. Virol. 89: 791-798 [Abstract] [Full Text]  
  • Long, G., Pan, X., Vlak, J. M. (2008). Conserved Leucines in N-Terminal Heptad Repeat HR1 of Envelope Fusion Protein F of Group II Nucleopolyhedroviruses Are Important for Correct Processing and Essential for Fusogenicity. J. Virol. 82: 2437-2447 [Abstract] [Full Text]  
  • Westenberg, M., Vlak, J. M. (2008). GP64 of group I nucleopolyhedroviruses cannot readily rescue infectivity of group II f-null nucleopolyhedroviruses. J. Gen. Virol. 89: 424-431 [Abstract] [Full Text]  
  • Zhou, J., Blissard, G. W. (2008). Display of Heterologous Proteins on gp64null Baculovirus Virions and Enhanced Budding Mediated by a Vesicular Stomatitis Virus G-Stem Construct. J. Virol. 82: 1368-1377 [Abstract] [Full Text]  
  • Westenberg, M., Uijtdewilligen, P., Vlak, J. M. (2007). Baculovirus envelope fusion proteins F and GP64 exploit distinct receptors to gain entry into cultured insect cells. J. Gen. Virol. 88: 3302-3306 [Abstract] [Full Text]  
  • Deng, F., Wang, R., Fang, M., Jiang, Y., Xu, X., Wang, H., Chen, X., Arif, B. M., Guo, L., Wang, H., Hu, Z. (2007). Proteomics Analysis of Helicoverpa armigera Single Nucleocapsid Nucleopolyhedrovirus Identified Two New Occlusion-Derived Virus-Associated Proteins, HA44 and HA100. J. Virol. 81: 9377-9385 [Abstract] [Full Text]  
  • Long, G., Pan, X., Vlak, J. M. (2007). Absence of N-linked glycans from the F2 subunit of the major baculovirus envelope fusion protein F enhances fusogenicity. J. Gen. Virol. 88: 441-449 [Abstract] [Full Text]  
  • Long, G., Pan, X., Westenberg, M., Vlak, J. M. (2006). Functional Role of the Cytoplasmic Tail Domain of the Major Envelope Fusion Protein of Group II Baculoviruses.. J. Virol. 80: 11226-11234 [Abstract] [Full Text]  
  • Long, G., Pan, X., Kormelink, R., Vlak, J. M. (2006). Functional entry of baculovirus into insect and Mammalian cells is dependent on clathrin-mediated endocytosis.. J. Virol. 80: 8830-8833 [Abstract] [Full Text]  
  • Lauzon, H. A. M., Garcia-Maruniak, A., de A. Zanotto, P. M., Clemente, J. C., Herniou, E. A., Lucarotti, C. J., Arif, B. M., Maruniak, J. E. (2006). Genomic comparison of Neodiprion sertifer and Neodiprion lecontei nucleopolyhedroviruses and identification of potential hymenopteran baculovirus-specific open reading frames. J. Gen. Virol. 87: 1477-1489 [Abstract] [Full Text]  
  • Long, G., Westenberg, M., Wang, H., Vlak, J. M., Hu, Z. (2006). Function, oligomerization and N-linked glycosylation of the Helicoverpa armigera single nucleopolyhedrovirus envelope fusion protein.. J. Gen. Virol. 87: 839-846 [Abstract] [Full Text]  
  • Vanarsdall, A. L., Okano, K., Rohrmann, G. F. (2006). Characterization of the Role of Very Late Expression Factor 1 in Baculovirus Capsid Structure and DNA Processing. J. Virol. 80: 1724-1733 [Abstract] [Full Text]  
  • Wu, D., Deng, F., Sun, X., Wang, H., Yuan, L., Vlak, J. M., Hu, Z. (2005). Functional analysis of FP25K of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus. J. Gen. Virol. 86: 2439-2444 [Abstract] [Full Text]  
  • Lung, O., Blissard, G. W. (2005). A Cellular Drosophila melanogaster Protein with Similarity to Baculovirus F Envelope Fusion Proteins. J. Virol. 79: 7979-7989 [Abstract] [Full Text]  
  • Liang, C., Song, J., Chen, X. (2005). The GP64 protein of Autographa californica multiple nucleopolyhedrovirus rescues Helicoverpa armigera nucleopolyhedrovirus transduction in mammalian cells. J. Gen. Virol. 86: 1629-1635 [Abstract] [Full Text]  
  • de Jong, J. G., Lauzon, H. A. M., Dominy, C., Poloumienko, A., Carstens, E. B., Arif, B. M., Krell, P. J. (2005). Analysis of the Choristoneura fumiferana nucleopolyhedrovirus genome. J. Gen. Virol. 86: 929-943 [Abstract] [Full Text]  
  • Lauzon, H. A. M., Jamieson, P. B., Krell, P. J., Arif, B. M. (2005). Gene organization and sequencing of the Choristoneura fumiferana defective nucleopolyhedrovirus genome. J. Gen. Virol. 86: 945-961 [Abstract] [Full Text]  
  • Westenberg, M., Veenman, F., Roode, E. C., Goldbach, R. W., Vlak, J. M., Zuidema, D. (2004). Functional Analysis of the Putative Fusion Domain of the Baculovirus Envelope Fusion Protein F. J. Virol. 78: 6946-6954 [Abstract] [Full Text]  
  • Lauzon, H. A. M., Lucarotti, C. J., Krell, P. J., Feng, Q., Retnakaran, A., Arif, B. M. (2004). Sequence and Organization of the Neodiprion lecontei Nucleopolyhedrovirus Genome. J. Virol. 78: 7023-7035 [Abstract] [Full Text]  
  • Fang, M., Wang, H., Wang, H., Yuan, L., Chen, X., Vlak, J. M., Hu, Z. (2003). Open reading frame 94 of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus encodes a novel conserved occlusion-derived virion protein, ODV-EC43. J. Gen. Virol. 84: 3021-3027 [Abstract] [Full Text]  
  • Braunagel, S. C., Russell, W. K., Rosas-Acosta, G., Russell, D. H., Summers, M. D. (2003). Determination of the protein composition of the occlusion-derived virus of Autographa californica nucleopolyhedrovirus. Proc. Natl. Acad. Sci. USA 100: 9797-9802 [Abstract] [Full Text]  
  • Long, G., Chen, X., Peters, D., Vlak, J. M., Hu, Z. (2003). Open reading frame 122 of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus encodes a novel structural protein of occlusion-derived virions. J. Gen. Virol. 84: 115-121 [Abstract] [Full Text]  
  • Washburn, J. O., Chan, E. Y., Volkman, L. E., Aumiller, J. J., Jarvis, D. L. (2002). Early Synthesis of Budded Virus Envelope Fusion Protein GP64 Enhances Autographa californica Multicapsid Nucleopolyhedrovirus Virulence in Orally Infected Heliothis virescens. J. Virol. 77: 280-290 [Abstract] [Full Text]  
  • Lung, O. Y., Cruz-Alvarez, M., Blissard, G. W. (2002). Ac23, an Envelope Fusion Protein Homolog in the Baculovirus Autographa californica Multicapsid Nucleopolyhedrovirus, Is a Viral Pathogenicity Factor. J. Virol. 77: 328-339 [Abstract] [Full Text]  
  • Pearson, M. N., Rohrmann, G. F. (2002). Transfer, Incorporation, and Substitution of Envelope Fusion Proteins among Members of the Baculoviridae, Orthomyxoviridae, and Metaviridae (Insect Retrovirus) Families. J. Virol. 76: 5301-5304 [Full Text]  
  • Lung, O., Westenberg, M., Vlak, J. M., Zuidema, D., Blissard, G. W. (2002). Pseudotyping Autographa californica Multicapsid Nucleopolyhedrovirus (AcMNPV): F Proteins from Group II NPVs Are Functionally Analogous to AcMNPV GP64. J. Virol. 76: 5729-5736 [Abstract] [Full Text]  
  • Pearson, M. N., Russell, R. L. Q., Rohrmann, G. F. (2002). Transcriptional mapping of two genes encoding baculovirus envelope-associated proteins. J. Gen. Virol. 83: 937-943 [Abstract] [Full Text]  
  • Hyink, O., Dellow, R. A., Olsen, M. J., Caradoc-Davies, K. M. B., Drake, K., Herniou, E. A., Cory, J. S., O'Reilly, D. R., Ward, V. K. (2002). Whole genome analysis of the Epiphyas postvittana nucleopolyhedrovirus. J. Gen. Virol. 83: 957-971 [Abstract] [Full Text]  
  • Westenberg, M., Wang, H., IJkel, W. F. J., Goldbach, R. W., Vlak, J. M., Zuidema, D. (2002). Furin Is Involved in Baculovirus Envelope Fusion Protein Activation. J. Virol. 76: 178-184 [Abstract] [Full Text]  
  • Afonso, C. L., Tulman, E. R., Lu, Z., Balinsky, C. A., Moser, B. A., Becnel, J. J., Rock, D. L., Kutish, G. F. (2001). Genome Sequence of a Baculovirus Pathogenic for Culex nigripalpus. J. Virol. 75: 11157-11165 [Abstract] [Full Text]  
  • Luque, T., Finch, R., Crook, N., O'Reilly, D. R., Winstanley, D. (2001). The complete sequence of the Cydia pomonella granulovirus genome. J. Gen. Virol. 82: 2531-2547 [Abstract] [Full Text]  
  • Herniou, E. A., Luque, T., Chen, X., Vlak, J. M., Winstanley, D., Cory, J. S., O'Reilly, D. R. (2001). Use of Whole Genome Sequence Data To Infer Baculovirus Phylogeny. J. Virol. 75: 8117-8126 [Abstract] [Full Text]  
  • Mangor, J. T., Monsma, S. A., Johnson, M. C., Blissard, G. W. (2001). A GP64-Null Baculovirus Pseudotyped with Vesicular Stomatitis Virus G Protein. J. Virol. 75: 2544-2556 [Abstract] [Full Text]  
  • Chen, X., IJkel, W. F. J., Tarchini, R., Sun, X., Sandbrink, H., Wang, H., Peters, S., Zuidema, D., Lankhorst, R. K., Vlak, J. M., Hu, Z. (2001). The sequence of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus genome. J. Gen. Virol. 82: 241-257 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»