This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yeh, H.-H.
Right arrow Articles by Falk, B. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yeh, H.-H.
Right arrow Articles by Falk, B. W.

 Previous Article  |  Next Article 

Journal of Virology, July 2000, p. 5762-5768, Vol. 74, No. 13
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Asynchronous Accumulation of Lettuce Infectious Yellows Virus RNAs 1 and 2 and Identification of an RNA 1 trans Enhancer of RNA 2 Accumulation

Hsin-Hung Yeh, Tongyan Tian, Luis Rubio, Brett Crawford, and Bryce W. Falk*

Department of Plant Pathology, University of California, Davis, California 95616

Received 28 January 2000/Accepted 17 April 2000

Time course and mutational analyses were used to examine the accumulation in protoplasts of progeny RNAs of the bipartite Crinivirus, Lettuce infectious yellow virus (LIYV; family Closteroviridae). Hybridization analyses showed that simultaneous inoculation of LIYV RNAs 1 and 2 resulted in asynchronous accumulation of progeny LIYV RNAs. LIYV RNA 1 progeny genomic and subgenomic RNAs could be detected in protoplasts as early as 12 h postinoculation (p.i.) and accumulated to high levels by 24 h p.i. The LIYV RNA 1 open reading frame 2 (ORF 2) subgenomic RNA was the most abundant of all LIYV RNAs detected. In contrast, RNA 2 progeny were not readily detected until ca. 36 h p.i. Mutational analyses showed that in-frame stop codons introduced into five of seven RNA 2 ORFs did not affect accumulation of progeny LIYV RNA 1 or RNA 2, confirming that RNA 2 does not encode proteins necessary for LIYV RNA replication. Mutational analyses also supported that LIYV RNA 1 encodes proteins necessary for replication of LIYV RNAs 1 and 2. A mutation introduced into the LIYV RNA 1 region encoding the overlapping ORF 1B and ORF 2 was lethal. However, mutations introduced into only LIYV RNA 1 ORF 2 resulted in accumulation of progeny RNA 1 near or equal to wild-type RNA 1. In contrast, the RNA 1 ORF 2 mutants did not efficiently support the trans accumulation of LIYV RNA 2. Three distinct RNA 1 ORF 2 mutants were analyzed and all exhibited a similar phenotype for progeny LIYV RNA accumulation. These data suggest that the LIYV RNA 1 ORF 2 encodes a trans enhancer for RNA 2 accumulation.

* Corresponding author. Mailing address: Department of Plant Pathology, 1 Shields Ave., University of California, Davis, CA 95616. Phone: (530) 752-0302. Fax: (530) 752-5674. E-mail: bwfalk{at}ucdavis.edu.

Journal of Virology, July 2000, p. 5762-5768, Vol. 74, No. 13
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lozano, G., Grande-Perez, A., Navas-Castillo, J. (2009). Populations of Genomic RNAs Devoted to the Replication or Spread of a Bipartite Plant Virus Differ in Genetic Structure. J. Virol. 83: 12973-12983 [Abstract] [Full Text]  
  • Lu, H.-C., Chen, H.-H., Tsai, W.-C., Chen, W.-H., Su, H.-J., Chang, D. C.-N., Yeh, H.-H. (2007). Strategies for Functional Validation of Genes Involved in Reproductive Stages of Orchids. Plant Physiol. 143: 558-569 [Abstract] [Full Text]  
  • Ng, J. C. K., Tian, T., Falk, B. W. (2004). Quantitative parameters determining whitefly (Bemisia tabaci) transmission of Lettuce infectious yellows virus and an engineered defective RNA. J. Gen. Virol. 85: 2697-2707 [Abstract] [Full Text]  
  • Livieratos, I. C., Eliasco, E., Muller, G., Olsthoorn, R. C. L., Salazar, L. F., Pleij, C. W. A., Coutts, R. H. A. (2004). Analysis of the RNA of Potato yellow vein virus: evidence for a tripartite genome and conserved 3'-terminal structures among members of the genus Crinivirus. J. Gen. Virol. 85: 2065-2075 [Abstract] [Full Text]  
  • Kreuze, J. F., Savenkov, E. I., Valkonen, J. P. T. (2002). Complete Genome Sequence and Analyses of the Subgenomic RNAs of Sweet Potato Chlorotic Stunt Virus Reveal Several New Features for the Genus Crinivirus. J. Virol. 76: 9260-9270 [Abstract] [Full Text]  
  • Qiu, W., Scholthof, H. B. (2001). Effects of inactivation of the coat protein and movement genes of Tomato bushy stunt virus on early accumulation of genomic and subgenomic RNAs. J. Gen. Virol. 82: 3107-3114 [Abstract] [Full Text]  
  • Erokhina, T. N., Vitushkina, M. V., Zinovkin, R. A., Lesemann, D. E., Jelkmann, W., Koonin, E. V., Agranovsky, A. A. (2001). Ultrastructural localization and epitope mapping of the methyltransferase-like and helicase-like proteins of Beet yellows virus. J. Gen. Virol. 82: 1983-1994 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»