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Journal of Virology, June 2000, p. 5726-5728, Vol. 74, No. 12
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Unwinding of a Herpes Simplex Virus Type 1 Origin of Replication
(OriS) by a Complex of the Viral Origin Binding Protein
and the Single-Stranded DNA Binding Protein
Xiaodun
He and
I. R.
Lehman*
Department of Biochemistry, Stanford
University, Stanford, California 94305-5037
Received 14 February 2000/Accepted 29 March 2000
A herpes simplex virus type 1 (HSV-1) OriS analogue in
which the A+T sequence linking the box I and II elements was replaced by two single-stranded oligo(dT)s is unwound by the UL9 protein-ICP8 complex. Unwinding of wild-type OriS by the UL9
protein-ICP8 complex was also observed under conditions which
destabilize the A+T sequence. These experiments support a model for the
unwinding of OriS in which destabilization of the A+T
sequence can generate a single-stranded DNA binding site for ICP8,
which then associates with the UL9 protein bound to boxes I and II to
promote the bidirectional unwinding of OriS.
*
Corresponding author. Mailing address: Department of
Biochemistry, Beckman Center, Stanford University, Stanford, CA
94305-5037. Phone: (650) 723-6164. Fax: (650) 723-6783. E-mail:
blehman{at}cmgm.stanford.edu.
Journal of Virology, June 2000, p. 5726-5728, Vol. 74, No. 12
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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