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Journal of Virology, June 2000, p. 5709-5711, Vol. 74, No. 12
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Application of the Intracellular Gamma Interferon Assay To Recalculate the Potency of CD8+ T-Cell Responses to Herpes Simplex Virus

Udayasankar Kumaraguru and Barry T. Rouse*

Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996-0845

Received 2 February 2000/Accepted 24 March 2000

Enumeration and characterization of herpes simplex virus (HSV)-specific CD8+ T lymphocytes are tedious and indirect. We quantitated antigen-specific CD8+ T cells during acute and secondary stages of HSV infection using intracellular gamma interferon production upon stimulation with virus or immunodominant peptide. Results show a substantial increase in the number of CD8+ T cells which was otherwise underestimated with the conventional limiting dilution analysis.

* Corresponding author. Mailing address: M409 Walters Life Sciences Bldg., University of Tennessee, Knoxville, TN 37996-0845. Phone: (865) 974-4026. Fax: (865) 974-4007. E-mail: btr{at}utk.edu.

Journal of Virology, June 2000, p. 5709-5711, Vol. 74, No. 12
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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