This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Agol, V. I.
Right arrow Articles by Tolskaya, E. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Agol, V. I.
Right arrow Articles by Tolskaya, E. A.

 Previous Article  |  Next Article 

Journal of Virology, June 2000, p. 5534-5541, Vol. 74, No. 12
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Competing Death Programs in Poliovirus-Infected Cells: Commitment Switch in the Middle of the Infectious Cycle

Vadim I. Agol,1,2,* George A. Belov,1,2 Kurt Bienz,3 Denise Egger,3 Marina S. Kolesnikova,1 Lyudmila I. Romanova,1 Larissa V. Sladkova,4 and Elena A. Tolskaya1

M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences, Moscow Region 142782,1 M. V. Lomonosov Moscow State University, Moscow 119899,2 and Moscow Research Institute of Medical Ecology, Moscow 113149,4 Russia, and Institute for Medical Microbiology, University of Basel, CH-4003 Basel, Switzerland3

Received 16 November 1999/Accepted 18 March 2000

Productive poliovirus infection of HeLa cells leads to the canonical cytopathic effect (CPE), whereas certain types of abortive infection result in apoptosis. To define the time course of commitment to the different types of poliovirus-induced death, inhibitors of viral replication (guanidine HCl) or translation (cycloheximide) were added at different times postinfection (p.i.). Early in the infection (during the first ~2 h p.i.), predominantly proapoptotic viral function was expressed, rendering the cells committed to apoptosis, which developed several hours after viral expression was arrested. In the middle of infection, concomitantly with the onset of fast generation of viral progeny, the implementation of the viral apoptotic program was abruptly interrupted. In particular, activation of an Asp-Glu-Val-Asp (DEVD)-specific caspase(s) occurring in the apoptosis-committed cells was prevented by the ongoing productive infection. Simultaneously, the cells retaining normal or nearly normal morphology became committed to CPE, which eventually developed regardless of whether or not further viral expression was allowed to proceed. The implementation of the poliovirus-induced apoptotic program was suppressed in HeLa cells overexpressing the Bcl-2 protein, indicating that the fate of poliovirus-infected cells depends on the balance of host and viral pro- and antiapoptotic factors.

* Corresponding author. Mailing address: Institute of Poliomyelitis, Moscow Region 142782, Russia. Phone: 7 (095) 439 90 26. Fax: 7 (095) 439 93 21. E-mail: viago{at}ipive.genebee.msu.su.

Journal of Virology, June 2000, p. 5534-5541, Vol. 74, No. 12
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Romanova, L. I., Lidsky, P. V., Kolesnikova, M. S., Fominykh, K. V., Gmyl, A. P., Sheval, E. V., Hato, S. V., van Kuppeveld, F. J. M., Agol, V. I. (2009). Antiapoptotic Activity of the Cardiovirus Leader Protein, a Viral "Security" Protein. J. Virol. 83: 7273-7284 [Abstract] [Full Text]  
  • Autret, A., Martin-Latil, S., Brisac, C., Mousson, L., Colbere-Garapin, F., Blondel, B. (2008). Early Phosphatidylinositol 3-Kinase/Akt Pathway Activation Limits Poliovirus-Induced JNK-Mediated Cell Death. J. Virol. 82: 3796-3802 [Abstract] [Full Text]  
  • Autret, A., Martin-Latil, S., Mousson, L., Wirotius, A., Petit, F., Arnoult, D., Colbere-Garapin, F., Estaquier, J., Blondel, B. (2007). Poliovirus Induces Bax-Dependent Cell Death Mediated by c-Jun NH2-Terminal Kinase. J. Virol. 81: 7504-7516 [Abstract] [Full Text]  
  • Han, J.-Q., Townsend, H. L., Jha, B. K., Paranjape, J. M., Silverman, R. H., Barton, D. J. (2007). A Phylogenetically Conserved RNA Structure in the Poliovirus Open Reading Frame Inhibits the Antiviral Endoribonuclease RNase L. J. Virol. 81: 5561-5572 [Abstract] [Full Text]  
  • Labadie, K., Saulnier, A., Martin-Latil, S., Colbere-Garapin, F. (2007). Reduced Apoptosis in Human Intestinal Cells Cured of Persistent Poliovirus Infection. J. Virol. 81: 3033-3036 [Abstract] [Full Text]  
  • Salako, M. A., Carter, M. J., Kass, G. E. N. (2006). Coxsackievirus Protein 2BC Blocks Host Cell Apoptosis by Inhibiting Caspase-3. J. Biol. Chem. 281: 16296-16304 [Abstract] [Full Text]  
  • Neznanov, N., Chumakov, K. M., Neznanova, L., Almasan, A., Banerjee, A. K., Gudkov, A. V. (2005). Proteolytic Cleavage of the p65-RelA Subunit of NF-{kappa}B during Poliovirus Infection. J. Biol. Chem. 280: 24153-24158 [Abstract] [Full Text]  
  • Wahid, R., Cannon, M. J., Chow, M. (2005). Dendritic Cells and Macrophages Are Productively Infected by Poliovirus. J. Virol. 79: 401-409 [Abstract] [Full Text]  
  • Banerjee, R., Weidman, M. K., Echeverri, A., Kundu, P., Dasgupta, A. (2004). Regulation of Poliovirus 3C Protease by the 2C Polypeptide. J. Virol. 78: 9243-9256 [Abstract] [Full Text]  
  • Graham, K. L., Gustin, K. E., Rivera, C., Kuyumcu-Martinez, N. M., Choe, S. S., Lloyd, R. E., Sarnow, P., Utz, P. J. (2004). Proteolytic Cleavage of the Catalytic Subunit of DNA-Dependent Protein Kinase during Poliovirus Infection. J. Virol. 78: 6313-6321 [Abstract] [Full Text]  
  • Ahn, J., Choi, J., Joo, C. H., Seo, I., Kim, D., Yoon, S. Y., Kim, Y. K., Lee, H. (2004). Susceptibility of mouse primary cortical neuronal cells to coxsackievirus B. J. Gen. Virol. 85: 1555-1564 [Abstract] [Full Text]  
  • Campanella, M., de Jong, A. S., Lanke, K. W. H., Melchers, W. J. G., Willems, P. H. G. M., Pinton, P., Rizzuto, R., van Kuppeveld, F. J. M. (2004). The Coxsackievirus 2B Protein Suppresses Apoptotic Host Cell Responses by Manipulating Intracellular Ca2+ Homeostasis. J. Biol. Chem. 279: 18440-18450 [Abstract] [Full Text]  
  • Schlitt, B. P., Felrice, M., Jelachich, M. L., Lipton, H. L. (2003). Apoptotic Cells, Including Macrophages, Are Prominent in Theiler's Virus-Induced Inflammatory, Demyelinating Lesions. J. Virol. 77: 4383-4388 [Abstract] [Full Text]  
  • Belov, G. A., Romanova, L. I., Tolskaya, E. A., Kolesnikova, M. S., Lazebnik, Y. A., Agol, V. I. (2002). The Major Apoptotic Pathway Activated and Suppressed by Poliovirus. J. Virol. 77: 45-56 [Abstract] [Full Text]  
  • Gosselin, A.-S., Simonin, Y., Guivel-Benhassine, F., Rincheval, V., Vayssiere, J.-L., Mignotte, B., Colbere-Garapin, F., Couderc, T., Blondel, B. (2002). Poliovirus-Induced Apoptosis Is Reduced in Cells Expressing a Mutant CD155 Selected during Persistent Poliovirus Infection in Neuroblastoma Cells. J. Virol. 77: 790-798 [Abstract] [Full Text]  
  • Couderc, T., Guivel-Benhassine, F., Calaora, V., Gosselin, A.-S., Blondel, B. (2002). An ex vivo murine model to study poliovirus-induced apoptosis in nerve cells. J. Gen. Virol. 83: 1925-1930 [Abstract] [Full Text]  
  • Gustin, K. E., Sarnow, P. (2002). Inhibition of Nuclear Import and Alteration of Nuclear Pore Complex Composition by Rhinovirus. J. Virol. 76: 8787-8796 [Abstract] [Full Text]  
  • Kuo, R.-L., Kung, S.-H., Hsu, Y.-Y., Liu, W.-T. (2002). Infection with enterovirus 71 or expression of its 2A protease induces apoptotic cell death. J. Gen. Virol. 83: 1367-1376 [Abstract] [Full Text]  
  • Zamora, M., Marissen, W. E., Lloyd, R. E. (2002). Multiple eIF4GI-Specific Protease Activities Present in Uninfected and Poliovirus-Infected Cells. J. Virol. 76: 165-177 [Abstract] [Full Text]  
  • Koyama, A. H., Irie, H., Ueno, F., Ogawa, M., Nomoto, A., Adachi, A. (2001). Suppression of apoptotic and necrotic cell death by poliovirus. J. Gen. Virol. 82: 2965-2972 [Abstract] [Full Text]  
  • Neznanov, N., Kondratova, A., Chumakov, K. M., Angres, B., Zhumabayeva, B., Agol, V. I., Gudkov, A. V. (2001). Poliovirus Protein 3A Inhibits Tumor Necrosis Factor (TNF)-Induced Apoptosis by Eliminating the TNF Receptor from the Cell Surface. J. Virol. 75: 10409-10420 [Abstract] [Full Text]  
  • Jelachich, M. L., Lipton, H. L. (2001). Theiler's Murine Encephalomyelitis Virus Induces Apoptosis in Gamma Interferon-Activated M1 Differentiated Myelomonocytic Cells through a Mechanism Involving Tumor Necrosis Factor Alpha (TNF-{alpha}) and TNF-{alpha}-Related Apoptosis-Inducing Ligand. J. Virol. 75: 5930-5938 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»