This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Azoulay-Cayla, A.
Right arrow Articles by Bureau, J.-F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Azoulay-Cayla, A.
Right arrow Articles by Bureau, J.-F.

 Previous Article  |  Next Article 

Journal of Virology, June 2000, p. 5470-5476, Vol. 74, No. 12
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

H-2Dbminus /minus Mice Are Susceptible to Persistent Infection by Theiler's Virus

Arièle Azoulay-Cayla,1,dagger Sven Dethlefs,1,Dagger Béatrice Pérarnau,2,|| Eva-Lotta Larsson-Sciard,1,§ François A. Lemonnier,2 Michel Brahic,1,* and Jean-François Bureau1

Unité des Virus Lents, CNRS URA 1930,1 and Unité d'Immunité Cellulaire Antivirale,2 Institut Pasteur, 75724 Paris Cedex 15, France

Received 8 November 1999/Accepted 20 March 2000

H-2b mice are resistant to persistent infection of the central nervous system by Theiler's virus. They clear the infection 7 to 10 days after intracranial inoculation. Resistance maps to the H-2D gene and not to the H-2K gene and is associated with a potent antiviral cytotoxic T-lymphocyte (CTL) response. We used H-2b mice in which the H-2D or the H-2K gene had been inactivated to dissect the respective roles of these genes in resistance. We report that H-2D-/- but not H-2K-/- mice were susceptible to persistent infection. Furthermore, whereas H-2K-/- mice mounted a vigorous virus-specific CTL response, similar to that of control C57BL/6 mice, the CTL response of H-2D-/- mice was nil or minimal. Using target cells transfected with the H-2Db or the H-2Kb gene, we showed that the H-2K-restricted CTL response against the virus was minimal in H-2D-/- mice. These results demonstrate that the H-2Db and H-2Kb genes play nonredundant roles in the resistance to this persistent infection.

* Corresponding author. Mailing address: Unité des Virus Lents, CNRS URA 1930, Institut Pasteur, 28, rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: 33(0)1 45 68 87 70. Fax: 33(0)1 40 61 31 67. E-mail: mbrahic{at}pasteur.fr.

dagger Present address: Fédération de Neurologie, Hôpital de la Pitié-Salpêtrière, 75651 Paris Cedex 13, France.

Dagger Present address: McKinsey & Company, Inc., 80538 Munich, Germany.

§ Present address: Department of Immunology, Division of Investigative Science, ICSM Chelsea & Westminster Hospital, London SW10 9NH, United Kingdom.

|| Present address: Centre d'Immunologie de Marseille-Luminy, 13288 Marseille Cedex 9, France.

Journal of Virology, June 2000, p. 5470-5476, Vol. 74, No. 12
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Levillayer, F., Mas, M., Levi-Acobas, F., Brahic, M., Bureau, J. F. (2007). Interleukin 22 Is a Candidate Gene for Tmevp3, a Locus Controlling Theiler's Virus-Induced Neurological Diseases. Genetics 176: 1835-1844 [Abstract] [Full Text]  
  • Getts, M. T., Kim, B. S., Miller, S. D. (2007). Differential Outcome of Tolerance Induction in Naive versus Activated Theiler's Virus Epitope-Specific CD8+ Cytotoxic T Cells. J. Virol. 81: 6584-6593 [Abstract] [Full Text]  
  • He, Y., Reichow, S., Ramamoorthy, S., Ding, X., Lathigra, R., Craig, J. C., Sobral, B. W. S., Schurig, G. G., Sriranganathan, N., Boyle, S. M. (2006). Brucella melitensis Triggers Time-Dependent Modulation of Apoptosis and Down-Regulation of Mitochondrion-Associated Gene Expression in Mouse Macrophages. Infect. Immun. 74: 5035-5046 [Abstract] [Full Text]  
  • Mendez-Fernandez, Y. V., Hansen, M. J., Rodriguez, M., Pease, L. R. (2005). Anatomical and Cellular Requirements for the Activation and Migration of Virus-Specific CD8+ T Cells to the Brain during Theiler's Virus Infection. J. Virol. 79: 3063-3070 [Abstract] [Full Text]  
  • Vigneau, S., Rohrlich, P.-S., Brahic, M., Bureau, J.-F. (2003). Tmevpg1, a Candidate Gene for the Control of Theiler's Virus Persistence, Could Be Implicated in the Regulation of Gamma Interferon. J. Virol. 77: 5632-5638 [Abstract] [Full Text]  
  • Massa, P. T., Ropka, S. L., Saha, S., Fecenko, K. L., Beuler, K. L. (2002). Critical Role for Protein Tyrosine Phosphatase SHP-1 in Controlling Infection of Central Nervous System Glia and Demyelination by Theiler's Murine Encephalomyelitis Virus. J. Virol. 76: 8335-8346 [Abstract] [Full Text]  
  • Aubagnac, S., Brahic, M., Bureau, J.-F. (2002). Bone Marrow Chimeras Reveal Non-H-2 Hematopoietic Control of Susceptibility to Theiler's Virus Persistent Infection. J. Virol. 76: 5807-5812 [Abstract] [Full Text]  
  • Lyman, M. A., Lee, H.-G., Kang, B. S., Kang, H.-K., Kim, B. S. (2002). Capsid-Specific Cytotoxic T Lymphocytes Recognize Three Distinct H-2Db-Restricted Regions of the BeAn Strain of Theiler's Virus and Exhibit Different Cytokine Profiles. J. Virol. 76: 3125-3134 [Abstract] [Full Text]  
  • Thach, D. C., Kleeberger, S. R., Tucker, P. C., Griffin, D. E. (2001). Genetic Control of Neuroadapted Sindbis Virus Replication in Female Mice Maps to Chromosome 2 and Associates with Paralysis and Mortality. J. Virol. 75: 8674-8680 [Abstract] [Full Text]  
  • Aubagnac, S., Brahic, M., Bureau, J.-F. (2001). Viral Load Increases in SJL/J Mice Persistently Infected by Theiler's Virus after Inactivation of the {beta}2m Gene. J. Virol. 75: 7723-7726 [Abstract] [Full Text]  
  • Azoulay-Cayla, A., Syan, S., Brahic, M., Bureau, J.-F. (2001). Roles of the H-2Db and H-Kb genes in resistance to persistent Theiler's murine encephalomyelitis virus infection of the central nervous system. J. Gen. Virol. 82: 1043-1047 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»