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Journal of Virology, May 2000, p. 4933-4937, Vol. 74, No. 10
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Distinct Mechanisms of Entry by Envelope Glycoproteins of Marburg and Ebola (Zaire) Viruses

Stephen Y. Chan,1,2 Roberto F. Speck,1,dagger Melissa C. Ma,1 and Mark A. Goldsmith1,2,*

Gladstone Institute of Virology and Immunology1 and University of California San Francisco,2 San Francisco, California 94141-9100

Received 6 May 1999/Accepted 11 February 2000

Since the Marburg (MBG) and Ebola (EBO) viruses have sequence homology and cause similar diseases, we hypothesized that they associate with target cells by similar mechanisms. Pseudotype viruses prepared with a luciferase-containing human immunodeficiency virus type 1 backbone and packaged by the MBG virus or the Zaire subtype EBO virus glycoproteins (GP) mediated infection of a comparable wide range of mammalian cell types, and both were inhibited by ammonium chloride. In contrast, they exhibited differential sensitivities to treatment of target cells with tunicamycin, endoglycosidase H, or protease (pronase). Therefore, while they exhibit certain functional similarities, the MBG and EBO virus GP interact with target cells by distinct processes.

* Corresponding author. Mailing address: Gladstone Institute of Virology and Immunology, P.O. Box 419100, San Francisco, CA 94141-9100. Phone: (415) 695-3775. Fax: (415) 695-1364. Email: mgoldsmith{at}gladstone.ucsf.edu.

dagger Present address: University Hospital Zürich, Zürich, Switzerland CH-8091.

Journal of Virology, May 2000, p. 4933-4937, Vol. 74, No. 10
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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