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Journal of Virology, May 2000, p. 4898-4901, Vol. 74, No. 10
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

L* Protein of Theiler's Murine Encephalomyelitis Virus Is Required for Virus Growth in a Murine Macrophage-Like Cell Line

Masatsugu Obuchi,1 Jiro Yamamoto,2 Takato Odagiri,1 Mohammed Nasir Uddin,1 Hideaki Iizuka,2 and Yoshiro Ohara1,*

Departments of Microbiology1 and Neurosurgery,2 Kanazawa Medical University, Uchinada, Ishikawa 920-0293, Japan

Received 28 July 1999/Accepted 14 February 2000

We sought to confirm the importance of L* protein for growth of Theiler's murine encephalomyelitis virus (TMEV) in a macrophage-like cell line, J774-1. The protein is out of frame with the polyprotein and synthesized in DA but not GDVII subgroup strains of TMEV. A recombinant virus, DANCL*/GD, which substitutes the DA 5' noncoding and L* coding regions for the corresponding regions of GDVII and synthesizes L* protein, grew with little restriction in J774-1 cells. In contrast, another recombinant virus, DANCL*-1/GD, which has an ACG rather than an AUG as the starting codon of L* protein at nucleotide 1079, resulting in no synthesis of L* protein, did not grow well. No significant difference between the rates of adsorption to J774-1 cells of these viruses was observed. RNase protection assay demonstrated that DANCL*/GD viral RNA significantly increased, whereas only a minimal increase was observed for DANCL*-1/GD. The present study suggests that L* protein is required for virus growth in macrophages.

* Corresponding author. Mailing address: Department of Microbiology, Kanazawa Medical University, Uchinada, Ishikawa 920-0293, Japan. Phone: 81-76-286-2211, ext. 3011. Fax: 81-76-286-3961. E-mail: ohara{at}kanazawa-med.ac.jp.

Journal of Virology, May 2000, p. 4898-4901, Vol. 74, No. 10
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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