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Journal of Virology, May 2000, p. 4891-4893, Vol. 74, No. 10
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cell-Dependent Requirement of Human Immunodeficiency Virus Type 1 gp41 Cytoplasmic Tail for Env Incorporation into Virions

Hirofumi Akari, Tomoharu Fukumori, and Akio Adachi*

Department of Virology, The University of Tokushima School of Medicine, Tokushima 770-8503, Japan

Received 8 November 1999/Accepted 16 February 2000

Growth kinetics in lymphocytic H9 and M8166 cells of two mutants of human immunodeficiency virus type 1 (HIV-1) with deleted gp41 cytoplasmic tails were examined. While the mutant viruses designated CTdel-44 and CTdel-144 were able to grow in M8166 cells, they were unable to grow in H9 cells. Transfection and single-round infectivity assays demonstrated that they are defective in the early phase of viral replication in H9 cells. Analysis of the mutant virions revealed drastically reduced incorporation of Env gp120 (compared with the incorporation of wild-type virions) in H9 cells but normal incorporation in M8166 cells. These results indicate that the HIV-1 cytoplasmic tail of gp41 determines virus infectivity in a cell-dependent manner by affecting incorporation of Env into virions and suggest the involvement of a host cell factor(s) in the Env incorporation.

* Corresponding author. Mailing address: Department of Virology, The University of Tokushima School of Medicine, 3 Kuramoto, Tokushima 770-8503, Japan. Phone: 81-88-633-7078. Fax: 81-88-633-7080. E-mail: adachi{at}basic.med.tokushima-u.ac.jp.

Journal of Virology, May 2000, p. 4891-4893, Vol. 74, No. 10
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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