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Journal of Virology, January 2000, p. 580-583, Vol. 74, No. 1
0022-538X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification of a Bovine Coronavirus Packaging Signal

Raymond Cologna1 and Brenda G. Hogue1,2,*

Division of Molecular Virology1 and Department of Microbiology and Immunology,2 Baylor College of Medicine, Houston, Texas 77030

Received 9 July 1999/Accepted 29 September 1999

A region of the bovine coronavirus (BCV) genome that functions as a packaging signal has been cloned. The 291-nucleotide clone shares 72% homology with the region of mouse hepatitis coronavirus (MHV) gene 1b that contains the packaging signal. RNA transcripts were packaged into both BCV and MHV virions when the cloned region was appended to a noncoronavirus RNA. This is the first identification of a BCV packaging signal. The data demonstrate that the BCV genome contains a sequence that is conserved at both the sequence and functional levels, thus broadening our insight into coronavirus packaging.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030. Phone: (713) 798-6412. Fax: (713) 798-7375. E-mail: bhogue{at}bcm.tmc.edu.

Journal of Virology, January 2000, p. 580-583, Vol. 74, No. 1
0022-538X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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