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Journal of Virology, January 2000, p. 552-555, Vol. 74, No. 1
0022-538X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Immune Complexes Containing Human Immunodeficiency Virus Type 1 Primary Isolates Bind to Lymphoid Tissue B Lymphocytes and Are Infectious for T Lymphocytes

Jocelyn J. Jakubik, Mohammed Saifuddin, Daniel M. Takefman, and Gregory T. Spear*

Department of Immunology/Microbiology, Rush University, Chicago, Illinois 60612

Received 23 June 1999/Accepted 22 September 1999

This study investigated the interaction of tonsil B lymphocytes with immune complexes containing human immunodeficiency virus (HIV IC) primary isolates and the infectivity of the B cell-bound HIV IC. Treatment of virus with a source of antibody and complement increased HIV IC binding to B cells by 5.6-fold. Most of the HIV IC that bound to B cells were not internalized but remained on the cell surface and were gradually released over 72 h. Cell-bound HIV IC were highly infectious for T cells while virus released by cultured B cells was only slightly infectious. Removal of HIV IC from the B-cell surface by protease treatment reduced the infection of T cells to near-background levels, indicating that infectious virus remained on the B-cell surface. These studies show that B lymphocytes can carry and transfer infectious HIV IC to T cells and thus suggest a novel mode of infection of T cells in lymphoid tissue that could be important for pathogenesis during HIV infection.

* Corresponding author. Mailing address: Department of Immunology/Microbiology, Rush University, 1653 W. Congress Pkwy., Chicago, IL 60612. Phone: (312) 942-2083. Fax: (312) 942-2808. E-mail: gspear{at}rush.edu

Journal of Virology, January 2000, p. 552-555, Vol. 74, No. 1
0022-538X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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