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Journal of Virology, January 2000, p. 535-540, Vol. 74, No. 1
0022-538X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Incorporation of Adeno-Associated Virus in a Calcium Phosphate Coprecipitate Improves Gene Transfer to Airway Epithelia In Vitro and In Vivo

Robert W. Walters,1,2 Dongsheng Duan,3 John F. Engelhardt,3,4 and Michael J. Welsh1,4,*

Howard Hughes Medical Institute,1 Departments of Internal Medicine,4 Physiology and Biophysics,2 and Anatomy and Cell Biology,3 University of Iowa College of Medicine, Iowa City, Iowa 52242

Received 28 June 1999/Accepted 30 September 1999

Adeno-associated virus (AAV) is inefficient at infecting differentiated airway epithelia because of a lack of receptors at the apical surface. We hypothesized that incorporation of AAV in a calcium phosphate coprecipitate would circumvent this barrier. Interestingly, coprecipitation of AAV type 2 improved gene transfer to differentiated human airway epithelia in vitro and to the mouse lung in vivo. These results suggest that delivery of AAV as a CaPi coprecipitate may significantly enhance its utility for gene transfer to the airway epithelia in vivo.

* Corresponding author. Mailing address: Howard Hughes Medical Institute, 500 EMRB, University of Iowa College of Medicine, Iowa City, IA 52242. Phone: (319) 335-7619. Fax: (319) 335-7623. E-mail: mjwelsh{at}blue.weeg.uiowa.edu.

Journal of Virology, January 2000, p. 535-540, Vol. 74, No. 1
0022-538X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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