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Journal of Virology, January 2000, p. 523-528, Vol. 74, No. 1
0022-538X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Small Dense Nuclear Bodies Are the Site of Localization of Herpes Simplex Virus 1 UL3 and UL4 Proteins and of ICP22 Only When the Latter Protein Is Present

Nancy S. Markovitz and Bernard Roizman*

The Marjorie B. Kovler Viral Oncology Laboratories, The University of Chicago, Chicago, Illinois 60637

Received 5 August 1999/Accepted 1 October 1999

The herpes simplex virus 1 UL3 and UL4 open reading frames are expressed late in infection and are not essential for viral replication in cultured cells in vitro. An earlier report showed that the UL4 protein colocalizes with the products of the alpha 22/US1.5 genes in small nuclear dense bodies. Here we report that the UL3 protein also colocalized in these small nuclear dense bodies and the localization of UL3 and UL4 proteins in these bodies required the presence of alpha 22/US1.5 genes. In cells infected with a mutant lacking intact alpha 22/US1.5 genes, UL3 was diffused throughout the nucleus even though the overall accumulation of the gamma 2 UL3 protein was decreased. The results suggest that ICP22 acts both as a regulator of UL3 accumulation and as the structural component and anchor of these small dense nuclear bodies.

* Corresponding author. Mailing address: The Marjorie B. Kovler Viral Oncology Laboratories, The University of Chicago, 910 E. 58th St., Chicago, IL 60637. Phone: (773) 702-1898. Fax: (773) 702-1631. E-mail: bernard{at}cummings.uchicago.edu.

Journal of Virology, January 2000, p. 523-528, Vol. 74, No. 1
0022-538X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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