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Journal of Virology, January 2000, p. 518-522, Vol. 74, No. 1
0022-538X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Avian Reticuloendotheliosis Virus Strain A and Spleen Necrosis Virus Do Not Infect Human Cells

R. Gautier,1 A. Jiang,2 V. Rousseau,3 R. Dornburg,2 and T. Jaffredo1,*

Institut d'Embryologie Cellulaire et Moléculaire du CNRS et du Collège de France, 94736 Nogent-sur-Marne Cedex,1 and Institut Alfred FESSARD-CNRS UPR 2212, 91198 Gif sur Yvette Cedex,3 France, and Division of Infectious Diseases, Center for Human Retrovirology, Thomas Jefferson University, Philadelphia, Pennsylvania 191072

Received 24 November 1998/Accepted 30 September 1999

Spleen necrosis virus (SNV) and Reticuloendotheliosis virus strain A (REV-A) belong to the family of reticuloendotheliosis viruses and are 90% sequence related. SNV-derived retroviral vectors produced by the REV-A-based D17.2G packaging cell line were shown to infect human cells (H.-M. Koo, A. M. C. Brown, Y. Ron, and J. P. Dougherty, J. Virol. 65:4769-4776, 1991), while similar vectors produced by another SNV-based packaging cell line, DSH134G, are not infectious in human cells (reviewed by R. Dornburg, Gene Ther. 2:301-310, 1995). Here we describe a careful reevaluation of the infectivity of vectors produced from the most commonly used REV-A- or SNV-based packaging cells obtained from various sources with, among them, one batch of D17.2G packaging cells obtained from the American Type Culture Collection. None of these packaging cells produced vectors able to infect human cells. Thus, contrary to previously published data, we conclude that REV-based vectors are not infectious in human cells.

* Corresponding author. Mailing address: Institut d'Embryologie Cellulaire et Moleculaire du CNRS et du College de France-UPR 9064, 49 bis Av. de la Belle Gabrielle, 94736 Nogent-sur-Marne Cedex, France. Phone: (1) 45 14 15 30. Fax: (1) 48 73 43 77. E-mail: jaffredo{at}infobiogen.fr.

Journal of Virology, January 2000, p. 518-522, Vol. 74, No. 1
0022-538X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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