This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Von Seggern, D. J.
Right arrow Articles by Nemerow, G. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Von Seggern, D. J.
Right arrow Articles by Nemerow, G. R.

 Previous Article  |  Next Article 

Journal of Virology, January 2000, p. 354-362, Vol. 74, No. 1
0022-538X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Adenovirus Vector Pseudotyping in Fiber-Expressing Cell Lines: Improved Transduction of Epstein-Barr Virus-Transformed B Cells

Dan J. Von Seggern,1 Shuang Huang,1 Shonna Kaye Fleck,1 Susan C. Stevenson,2 and Glen R. Nemerow1,*

Department of Immunology, Scripps Research Institute, La Jolla, California 92037,1 and Genetic Therapy, Inc., Gaithersburg, Maryland 208782

Received 8 June 1999/Accepted 22 September 1999

While adenovirus (Ad) gene delivery vectors are useful in many gene therapy applications, their broad tropism means that they cannot be directed to a specific target cell. There are also a number of cell types involved in human disease which are not transducible with standard Ad vectors, such as Epstein-Barr virus (EBV)-transformed B lymphocytes. Adenovirus binds to host cells via the viral fiber protein, and Ad vectors have previously been retargeted by modifying the fiber gene on the viral chromosome. This requires that the modified fiber be able to bind to the cell in which the vector is grown, which prevents truly specific vector targeting. We previously reported a gene delivery system based on a fiber gene-deleted Ad type 5 (Ad5) vector (Ad5.beta gal.Delta F) and packaging cells that express the viral fiber protein. Expression of different fibers in packaging cells will allow Ad retargeting without modifying the viral chromosome. Importantly, fiber proteins which can no longer bind to the producer cells can also be used. Using this approach, we generated for the first time pseudotyped Ad5.beta gal.Delta F particles containing either the wild-type Ad5 fiber protein or a chimeric fiber with the receptor-binding knob domain of the Ad3 fiber. Particles equipped with the chimeric fiber bound to the Ad3 receptor rather than the coxsackievirus-adenovirus receptor protein used by Ad5. EBV-transformed B lymphocytes were infected efficiently by the Ad3-pseudotyped particles but poorly by virus containing the Ad5 fiber protein. The strategy described here represents a broadly applicable method for targeting gene delivery to specific cell types.

* Corresponding author. Mailing address: Department of Immunology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: (858) 784-8072. Fax: (858) 784-8472. E-mail: gnemerow{at}scripps.edu.

Journal of Virology, January 2000, p. 354-362, Vol. 74, No. 1
0022-538X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Short, J. J., Curiel, D. T. (2009). Oncolytic adenoviruses targeted to cancer stem cells. Molecular Cancer Therapeutics 8: 2096-2102 [Abstract] [Full Text]  
  • Belousova, N., Mikheeva, G., Gelovani, J., Krasnykh, V. (2008). Modification of Adenovirus Capsid with a Designed Protein Ligand Yields a Gene Vector Targeted to a Major Molecular Marker of Cancer. J. Virol. 82: 630-637 [Abstract] [Full Text]  
  • Hesse, A., Kosmides, D., Kontermann, R. E., Nettelbeck, D. M. (2007). Tropism Modification of Adenovirus Vectors by Peptide Ligand Insertion into Various Positions of the Adenovirus Serotype 41 Short-Fiber Knob Domain. J. Virol. 81: 2688-2699 [Abstract] [Full Text]  
  • Li, J., Lad, S., Yang, G., Luo, Y., Iacobelli-Martinez, M., Primus, F. J., Reisfeld, R. A., Li, E. (2006). Adenovirus Fiber Shaft Contains a Trimerization Element That Supports Peptide Fusion for Targeted Gene Delivery. J. Virol. 80: 12324-12331 [Abstract] [Full Text]  
  • Schoggins, J. W., Falck-Pedersen, E. (2006). Fiber and Penton Base Capsid Modifications Yield Diminished Adenovirus Type 5 Transduction and Proinflammatory Gene Expression with Retention of Antigen-Specific Humoral Immunity. J. Virol. 80: 10634-10644 [Abstract] [Full Text]  
  • Verhaagh, S., de Jong, E., Goudsmit, J., Lecollinet, S., Gillissen, G., de Vries, M., van Leuven, K., Que, I., Ouwehand, K., Mintardjo, R., Weverling, G. J., Radosevic, K., Richardson, J., Eloit, M., Lowik, C., Quax, P., Havenga, M. (2006). Human CD46-transgenic mice in studies involving replication-incompetent adenoviral type 35 vectors. J. Gen. Virol. 87: 255-265 [Abstract] [Full Text]  
  • Richardson, C., Brennan, P., Powell, M., Prince, S., Chen, Y.-H., Spiller, O. B., Rowe, M. (2005). Susceptibility of B lymphocytes to adenovirus type 5 infection is dependent upon both coxsackie-adenovirus receptor and {alpha}v{beta}5 integrin expression. J. Gen. Virol. 86: 1669-1679 [Abstract] [Full Text]  
  • Sohn, R. H., Deming, C. B., Johns, D. C., Champion, H. C., Bian, C., Gardner, K., Rade, J. J. (2005). Regulation of endothelial thrombomodulin expression by inflammatory cytokines is mediated by activation of nuclear factor-kappa B. Blood 105: 3910-3917 [Abstract] [Full Text]  
  • Mercier, G. T., Campbell, J. A., Chappell, J. D., Stehle, T., Dermody, T. S., Barry, M. A. (2004). A chimeric adenovirus vector encoding reovirus attachment protein {sigma}1 targets cells expressing junctional adhesion molecule 1. Proc. Natl. Acad. Sci. USA 101: 6188-6193 [Abstract] [Full Text]  
  • Vellinga, J., Rabelink, M. J. W. E., Cramer, S. J., van den Wollenberg, D. J. M., Van der Meulen, H., Leppard, K. N., Fallaux, F. J., Hoeben, R. C. (2004). Spacers Increase the Accessibility of Peptide Ligands Linked to the Carboxyl Terminus of Adenovirus Minor Capsid Protein IX. J. Virol. 78: 3470-3479 [Abstract] [Full Text]  
  • Korokhov, N., Mikheeva, G., Krendelshchikov, A., Belousova, N., Simonenko, V., Krendelshchikova, V., Pereboev, A., Kotov, A., Kotova, O., Triozzi, P. L., Aldrich, W. A., Douglas, J. T., Lo, K.-M., Banerjee, P. T., Gillies, S. D., Curiel, D. T., Krasnykh, V. (2003). Targeting of Adenovirus via Genetic Modification of the Viral Capsid Combined with a Protein Bridge. J. Virol. 77: 12931-12940 [Abstract] [Full Text]  
  • Belousova, N., Korokhov, N., Krendelshchikova, V., Simonenko, V., Mikheeva, G., Triozzi, P. L., Aldrich, W. A., Banerjee, P. T., Gillies, S. D., Curiel, D. T., Krasnykh, V. (2003). Genetically Targeted Adenovirus Vector Directed to CD40-Expressing Cells. J. Virol. 77: 11367-11377 [Abstract] [Full Text]  
  • Wu, E., Pache, L., Von Seggern, D. J., Mullen, T.-M., Mikyas, Y., Stewart, P. L., Nemerow, G. R. (2003). Flexibility of the Adenovirus Fiber Is Required for Efficient Receptor Interaction. J. Virol. 77: 7225-7235 [Abstract] [Full Text]  
  • Kawakami, Y., Li, H., Lam, J. T., Krasnykh, V., Curiel, D. T., Blackwell, J. L. (2003). Substitution of the Adenovirus Serotype 5 Knob with a Serotype 3 Knob Enhances Multiple Steps in Virus Replication. Cancer Res. 63: 1262-1269 [Abstract] [Full Text]  
  • Schoggins, J. W., Gall, J. G. D., Falck-Pedersen, E. (2002). Subgroup B and F Fiber Chimeras Eliminate Normal Adenovirus Type 5 Vector Transduction In Vitro and In Vivo. J. Virol. 77: 1039-1048 [Abstract] [Full Text]  
  • Kanerva, A., Mikheeva, G. V., Krasnykh, V., Coolidge, C. J., Lam, J. T., Mahasreshti, P. J., Barker, S. D., Straughn, M., Barnes, M. N., Alvarez, R. D., Hemminki, A., Curiel, D. T. (2002). Targeting Adenovirus to the Serotype 3 Receptor Increases Gene Transfer Efficiency to Ovarian Cancer Cells. Clin. Cancer Res. 8: 275-280 [Abstract] [Full Text]  
  • Israel, B. F., Pickles, R. J., Segal, D. M., Gerard, R. D., Kenney, S. C. (2001). Enhancement of Adenovirus Vector Entry into CD70-Positive B-Cell Lines by Using a Bispecific CD70-Adenovirus Fiber Antibody. J. Virol. 75: 5215-5221 [Abstract] [Full Text]  
  • Chiu, C. Y., Wu, E., Brown, S. L., Von Seggern, D. J., Nemerow, G. R., Stewart, P. L. (2001). Structural Analysis of a Fiber-Pseudotyped Adenovirus with Ocular Tropism Suggests Differential Modes of Cell Receptor Interactions. J. Virol. 75: 5375-5380 [Abstract] [Full Text]  
  • YOUNG, L S, MAUTNER, V (2001). The promise and potential hazards of adenovirus gene therapy. Gut 48: 733-736 [Full Text]  
  • Rea, D., Havenga, M. J. E., van den Assem, M., Sutmuller, R. P. M., Lemckert, A., Hoeben, R. C., Bout, A., Melief, C. J. M., Offringa, R. (2001). Highly Efficient Transduction of Human Monocyte-Derived Dendritic Cells with Subgroup B Fiber-Modified Adenovirus Vectors Enhances Transgene-Encoded Antigen Presentation to Cytotoxic T Cells. J. Immunol. 166: 5236-5244 [Abstract] [Full Text]  
  • Jakubczak, J. L., Rollence, M. L., Stewart, D. A., Jafari, J. D., Von Seggern, D. J., Nemerow, G. R., Stevenson, S. C., Hallenbeck, P. L. (2001). Adenovirus Type 5 Viral Particles Pseudotyped with Mutagenized Fiber Proteins Show Diminished Infectivity of Coxsackie B-Adenovirus Receptor-Bearing Cells. J. Virol. 75: 2972-2981 [Abstract] [Full Text]  
  • Miyazawa, N., Crystal, R. G., Leopold, P. L. (2001). Adenovirus Serotype 7 Retention in a Late Endosomal Compartment prior to Cytosol Escape Is Modulated by Fiber Protein. J. Virol. 75: 1387-1400 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»