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Journal of Virology, September 1999, p. 7886-7890, Vol. 73, No. 9
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of Inhibitory Mutants of Cauliflower mosaic virus Movement Protein Function after Expression in Insect Cells

C. L. Thomas and A. J. Maule*

John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom

Received 12 November 1998/Accepted 9 June 1999

Cauliflower mosaic virus (CaMV) encodes a movement protein (MP) which forms tubules in vivo and mediates the translocation of virus particles through plasmodesmata. The relationship between CaMV MP structure and function, in isolation from the complete virus infection, was studied by using MP expression in insect cells. The study allowed the MP domains necessary for tubule formation to be identified and potential MP-MP interactions to be investigated by using double infections with recombinant baculoviruses. Two MP domains which interfered with the ability of the wild-type MP to form tubules were identified. These mutant domains appeared to act as competitive, rather than dominant negative, inhibitors.

* Corresponding author. Mailing address: John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom. Phone: 44-1603-452571. Fax: 44-1603-456844. E-mail: andy.maule{at}bbsrc.ac.uk.

Journal of Virology, September 1999, p. 7886-7890, Vol. 73, No. 9
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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