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Journal of Virology, September 1999, p. 7823-7829, Vol. 73, No. 9
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Rapid Delivery of Foreign Genes into Plants by Direct Rub-Inoculation with Intact Plasmid DNA of a Tomato Bushy Stunt Virus Gene Vector

Herman B. Scholthof*

Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas 77843

Received 8 March 1999/Accepted 17 May 1999

Tomato bushy stunt virus (TBSV) cDNA, positioned between a modified cauliflower mosaic virus 35S promoter and the hepatitis delta virus antigenomic ribozyme with a downstream nopaline synthase gene polyadenylation signal, established infections upon rub-inoculation of plants with intact plasmids. Application of this methodology produced a TBSV DNA-based gene vector which yielded readily detectable levels of localized foreign gene expression in inoculated leaves. This is the first demonstration of an infectious DNA from a member of the Tombusviridae which permits rapid TBSV-mediated foreign-gene expression upon direct rub-inoculation of miniprep DNA onto a variety of plant species.

* Corresponding author. Mailing address: Department of Plant Pathology and Microbiology, Texas A&M University, Room 120, L. F. Peterson Building, College Station, TX 77843-2132. Phone: (409) 862-1495. Fax: (409) 845-6483. E-mail: herscho{at}acs.tamu.edu.

Journal of Virology, September 1999, p. 7823-7829, Vol. 73, No. 9
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • McCartney, A. W., Greenwood, J. S., Fabian, M. R., White, K. A., Mullen, R. T. (2005). Localization of the Tomato Bushy Stunt Virus Replication Protein p33 Reveals a Peroxisome-to-Endoplasmic Reticulum Sorting Pathway. Plant Cell 17: 3513-3531 [Abstract] [Full Text]  


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