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Journal of Virology, September 1999, p. 7671-7677, Vol. 73, No. 9
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
An Inducible Human Immunodeficiency Virus Type 1 (HIV-1) Vector Which Effectively Suppresses HIV-1 Replication
Dong Sung
An,
Kouki
Morizono,
Qi-Xiang
Li,
Si Hua
Mao,
Stephanie
Lu, and
Irvin S. Y.
Chen*
Departments of Microbiology & Immunology and
Medicine, UCLA School of Medicine, Los Angeles, California 90095
Received 15 January 1999/Accepted 14 June 1999
Recently, gene therapy vectors based upon the human
immunodeficiency virus type 1 (HIV-1) genome have been developed. Here, we create an HIV-1 vector which is defective for all HIV-1 genes, but
which maintains cis-acting elements required for efficient packaging, infection, and expression. In T cells transduced by this
vector, vector expression is low but efficiently induced following
HIV-1 infection. Remarkably, although the HIV-1 vector does not contain
specific anti-HIV-1 therapeutic genes, the presence of the vector alone
is sufficient to inhibit the spread of HIV-1 infection. The mechanism
of inhibition is likely to be at the level of competition for limiting
substrates required for either efficient packaging or reverse
transcription, thereby selecting against propagation of wild-type
HIV-1. These results provide proof of a concept for potential
application of a novel HIV-1 vector in HIV-1 disease.
*
Corresponding author. Mailing address: Departments of
Microbiology & Immunology and Medicine, UCLA School of Medicine, Los Angeles, CA 90095-1678. Phone: (310) 825-4793. Fax: (310) 794-7682. E-mail: rtaweesu{at}ucla.edu.
Journal of Virology, September 1999, p. 7671-7677, Vol. 73, No. 9
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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