This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McNeal, M. M.
Right arrow Articles by Ward, R. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McNeal, M. M.
Right arrow Articles by Ward, R. L.

 Previous Article  |  Next Article 

Journal of Virology, September 1999, p. 7565-7573, Vol. 73, No. 9
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Antibody-Dependent and -Independent Protection following Intranasal Immunization of Mice with Rotavirus Particles

Monica M. McNeal,1 Mary N. Rae,1 Judy A. Bean,2 and Richard L. Ward1,*

Division of Infectious Diseases1 and Department of Biostatistics,2 Children's Hospital Medical Center, Cincinnati, Ohio 45229

Received 12 January 1999/Accepted 25 May 1999

The ability to elicit protective immune responses after intranasal immunization with rotavirus particles, either with or without the attenuated Escherichia coli heat-labile enterotoxin LT(R192G) as an adjuvant, was examined in the adult mouse model. BALB/c mice were administered one or two inoculations of psoralen/UV-inactivated, triple-layered (tl) or double-layered (dl) purified rotavirus particles. Four weeks after immunization, mice were challenged with the murine rotavirus strain EDIM, and the shedding of rotavirus antigen was quantified. Rotaviruses used for immunization included EDIM and heterotypic simian (RRV), bovine (WC3), and human (89-12) strains. tl EDIM stimulated both systemic and intestinal rotavirus antibody responses and complete protection with as little as one 1-µg dose. Inclusion of LT(R192G) (10 µg) significantly increased rotavirus antibody responses and reduced antigen concentrations needed for full protection. Both dl EDIM and heterotypic dl and tl particles stimulated protection, but they did so less than tl EDIM at comparable concentrations, either with or without LT(R192G). When B-cell-deficient µMt mice were immunized with tl EDIM particles, protection was reduced to levels similar to those induced with dl EDIM and heterotypic particles in BALB/c mice. However, dl EDIM particles induced similar levels of protection in both mouse strains. The protection stimulated by tl or dl EDIM particles was not diminished by CD8 cell depletion prior to immunization in either strain of mice. These results indicate that tl EDIM induced immunity at least partially through responses to its outer capsid proteins, presumably by stimulation of serotype-specific neutralizing antibody. In contrast, the other particles stimulated protection primarily by an antibody-independent mechanism. Finally, depletion of CD8 cells had no effect on protection by either mechanism.

* Corresponding author. Mailing address: Children's Hospital Medical Center, Division of Infectious Diseases, 3333 Burnet Ave., CH-1, Cincinnati, OH 45229-3039. Phone: (513) 636-7628. Fax: (513) 636-7682. E-mail: wardd0{at}chmcc.org.

Journal of Virology, September 1999, p. 7565-7573, Vol. 73, No. 9
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Borghan, M. A., Mori, Y., El-Mahmoudy, A.-B., Ito, N., Sugiyama, M., Takewaki, T., Minamoto, N. (2007). Induction of nitric oxide synthase by rotavirus enterotoxin NSP4: implication for rotavirus pathogenicity. J. Gen. Virol. 88: 2064-2072 [Abstract] [Full Text]  
  • Smiley, K. L., McNeal, M. M., Basu, M., Choi, A. H.-C., Clements, J. D., Ward, R. L. (2007). Association of Gamma Interferon and Interleukin-17 Production in Intestinal CD4+ T Cells with Protection against Rotavirus Shedding in Mice Intranasally Immunized with VP6 and the Adjuvant LT(R192G). J. Virol. 81: 3740-3748 [Abstract] [Full Text]  
  • Allen, S. R., Jafri, M., Donnelly, B., McNeal, M., Witte, D., Bezerra, J., Ward, R., Tiao, G. M. (2007). Effect of Rotavirus Strain on the Murine Model of Biliary Atresia. J. Virol. 81: 1671-1679 [Abstract] [Full Text]  
  • VanCott, J. L., Prada, A. E., McNeal, M. M., Stone, S. C., Basu, M., Huffer, B. Jr., Smiley, K. L., Shao, M., Bean, J. A., Clements, J. D., Choi, A. H.-C., Ward, R. L. (2006). Mice Develop Effective but Delayed Protective Immune Responses When Immunized as Neonates either Intranasally with Nonliving VP6/LT(R192G) or Orally with Live Rhesus Rotavirus Vaccine Candidates.. J. Virol. 80: 4949-4961 [Abstract] [Full Text]  
  • Parez, N., Fourgeux, C., Mohamed, A., Dubuquoy, C., Pillot, M., Dehee, A., Charpilienne, A., Poncet, D., Schwartz-Cornil, I., Garbarg-Chenon, A. (2006). Rectal Immunization with Rotavirus Virus-Like Particles Induces Systemic and Mucosal Humoral Immune Responses and Protects Mice against Rotavirus Infection. J. Virol. 80: 1752-1761 [Abstract] [Full Text]  
  • Glynn, A., Roy, C. J., Powell, B. S., Adamovicz, J. J., Freytag, L. C., Clements, J. D. (2005). Protection against Aerosolized Yersinia pestis Challenge following Homologous and Heterologous Prime-Boost with Recombinant Plague Antigens. Infect. Immun. 73: 5256-5261 [Abstract] [Full Text]  
  • McNeal, M. M., Sestak, K., Choi, A. H.-C., Basu, M., Cole, M. J., Aye, P. P., Bohm, R. P., Ward, R. L. (2005). Development of a Rotavirus-Shedding Model in Rhesus Macaques, Using a Homologous Wild-Type Rotavirus of a New P Genotype. J. Virol. 79: 944-954 [Abstract] [Full Text]  
  • Schwartz-Cornil, I., Benureau, Y., Greenberg, H., Hendrickson, B. A., Cohen, J. (2002). Heterologous Protection Induced by the Inner Capsid Proteins of Rotavirus Requires Transcytosis of Mucosal Immunoglobulins. J. Virol. 76: 8110-8117 [Abstract] [Full Text]  
  • McNeal, M. M., VanCott, J. L., Choi, A. H. C., Basu, M., Flint, J. A., Stone, S. C., Clements, J. D., Ward, R. L. (2002). CD4 T Cells Are the Only Lymphocytes Needed To Protect Mice against Rotavirus Shedding after Intranasal Immunization with a Chimeric VP6 Protein and the Adjuvant LT(R192G). J. Virol. 76: 560-568 [Abstract] [Full Text]  
  • Fromantin, C., Jamot, B., Cohen, J., Piroth, L., Pothier, P., Kohli, E. (2001). Rotavirus 2/6 Virus-Like Particles Administered Intranasally in Mice, with or without the Mucosal Adjuvants Cholera Toxin and Escherichia coli Heat-Labile Toxin, Induce a Th1/Th2-Like Immune Response. J. Virol. 75: 11010-11016 [Abstract] [Full Text]  
  • Yuan, L., Iosef, C., Azevedo, M. S. P., Kim, Y., Qian, Y., Geyer, A., Nguyen, T. V., Chang, K.-O., Saif, L. J. (2001). Protective Immunity and Antibody-Secreting Cell Responses Elicited by Combined Oral Attenuated Wa Human Rotavirus and Intranasal Wa 2/6-VLPs with Mutant Escherichia coli Heat-Labile Toxin in Gnotobiotic Pigs. J. Virol. 75: 9229-9238 [Abstract] [Full Text]  
  • Choi, A. H. C., Basu, M., McNeal, M. M., Flint, J., VanCott, J. L., Clements, J. D., Ward, R. L. (2000). Functional Mapping of Protective Domains and Epitopes in the Rotavirus VP6 Protein. J. Virol. 74: 11574-11580 [Abstract] [Full Text]  
  • Yuan, L., Geyer, A., Hodgins, D. C., Fan, Z., Qian, Y., Chang, K.-O., Crawford, S. E., Parreño, V., Ward, L. A., Estes, M. K., Conner, M. E., Saif, L. J. (2000). Intranasal Administration of 2/6-Rotavirus-Like Particles with Mutant Escherichia coli Heat-Labile Toxin (LT-R192G) Induces Antibody-Secreting Cell Responses but Not Protective Immunity in Gnotobiotic Pigs. J. Virol. 74: 8843-8853 [Abstract] [Full Text]  
  • O'Neal, C. M., Harriman, G. R., Conner, M. E. (2000). Protection of the Villus Epithelial Cells of the Small Intestine from Rotavirus Infection Does Not Require Immunoglobulin A. J. Virol. 74: 4102-4109 [Abstract] [Full Text]  
  • Choi, A. H.-C., Basu, M., McNeal, M. M., Clements, J. D., Ward, R. L. (1999). Antibody-Independent Protection against Rotavirus Infection of Mice Stimulated by Intranasal Immunization with Chimeric VP4 or VP6 Protein. J. Virol. 73: 7574-7581 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»