This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vicenzi, E.
Right arrow Articles by Poli, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vicenzi, E.
Right arrow Articles by Poli, G.

 Previous Article  |  Next Article 

Journal of Virology, September 1999, p. 7515-7523, Vol. 73, No. 9
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Envelope-Dependent Restriction of Human Immunodeficiency Virus Type 1 Spreading in CD4+ T Lymphocytes: R5 but Not X4 Viruses Replicate in the Absence of T-Cell Receptor Restimulation

Elisa Vicenzi,1,* Paola Panina Bordignon,2 Priscilla Biswas,1 Andrea Brambilla,1 Chiara Bovolenta,1 Manuela Cota,1 Francesco Sinigaglia,2 and Guido Poli1

AIDS Immunopathogenesis Unit, DIBIT, San Raffaele Scientific Institute,1 and Roche Milano Ricerche,2 20132 Milan, Italy

Received 16 February 1999/Accepted 21 May 1999

The human immunodeficiency virus (HIV) replicates in activated CD4+ T lymphocytes. However, only CD4+ Th2 and Th0, but not Th1, CD4+ T-cell clones have been reported to efficiently support HIV-1 replication. This dichotomous pattern was further investigated in the present study in Th1, Th2, or Th0 cell lines derived from umbilical human cord blood and in T-cell clones obtained from the peripheral blood mononuclear cells (PBMC) of healthy adults. Both primary and laboratory-adapted HIV-1 strains with CCR5 as the exclusive entry coreceptor (R5 viruses) efficiently replicated in Th1, Th2, and Th0 cells. In sharp contrast, CXCR4-dependent (X4) viruses poorly replicated in both polarized and unpolarized CD4+ T cells, including adults' PBMC infected several days after mitogenic stimulation. Unlike the X4 HIV-1NL4-3, a chimera in which the env gene had been replaced with that of the R5 HIV-1NL(AD8), efficiently replicated in both Th1 and Th2 cells. This X4-dependent restriction of HIV replication was not explained by either the absence of functional CXCR4 on the cell surface or by the inefficient viral entry and reverse transcription. T-cell receptor stimulation by anti-CD3 monoclonal antibodies fully rescued X4 HIV-1 replication in both Th1 and Th2 cells, whereas it did not alter the extent and kinetics of R5 HIV-1 spreading. Thus, R5 HIVs show a replicative advantage in comparison to X4 viruses in their ability to efficiently propagate among suboptimally activated T lymphocytes, regardless of their polarized or unpolarized functional profiles. This observation may help to explain the absolute predominance of R5 HIVs over X4 viruses observed after viral transmission and during early-stage disease.

* Corresponding author. Mailing address: P2-P3 Laboratories, DIBIT, Via Olgettina 58, 20132 Milan, Italy. Phone: 39-02-2643-4908. Fax: 39-02-2643-4905. E-mail: vicenzi.elisa{at}hsr.it.

Journal of Virology, September 1999, p. 7515-7523, Vol. 73, No. 9
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Gurney, K. B., Uittenbogaart, C. H. (2006). Human Immunodeficiency Virus Persistence and Production in T-Cell Development. CVI 13: 1237-1245 [Abstract] [Full Text]  
  • Locher, C. P., Witt, S. A., Kassel, R., Dowell, N. L., Fujimura, S., Levy, J. A. (2005). Differential effects of R5 and X4 human immunodeficiency virus type 1 infection on CD4+ cell proliferation and activation. J. Gen. Virol. 86: 1171-1179 [Abstract] [Full Text]  
  • Fantuzzi, L., Spadaro, F., Vallanti, G., Canini, I., Ramoni, C., Vicenzi, E., Belardelli, F., Poli, G., Gessani, S. (2003). Endogenous CCL2 (monocyte chemotactic protein-1) modulates human immunodeficiency virus type-1 replication and affects cytoskeleton organization in human monocyte-derived macrophages. Blood 102: 2334-2337 [Abstract] [Full Text]  
  • Lin, Y.-L., Mettling, C., Portales, P., Reynes, J., Clot, J., Corbeau, P. (2002). Cell surface CCR5 density determines the postentry efficiency of R5 HIV-1 infection. Proc. Natl. Acad. Sci. USA 99: 15590-15595 [Abstract] [Full Text]  
  • Vicenzi, E., Panina-Bodignon, P., Vallanti, G., Di Lucia, P., Poli, G. (2002). Restricted replication of primary HIV-1 isolates using both CCR5 and CXCR4 in Th2 but not in Th1 CD4+ T cells. J. Leukoc. Biol. 72: 913-920 [Abstract] [Full Text]  
  • Losana, G., Bovolenta, C., Rigamonti, L., Borghi, I., Altare, F., Jouanguy, E., Forni, G., Casanova, J.-L., Sherry, B., Mengozzi, M., Trinchieri, G., Poli, G., Gerosa, F., Novelli, F. (2002). IFN-{gamma} and IL-12 differentially regulate CC-chemokine secretion and CCR5 expression in human T lymphocytes. J. Leukoc. Biol. 72: 735-742 [Abstract] [Full Text]  
  • Marozsan, A. J., Torre, V. S., Johnson, M., Ball, S. C., Cross, J. V., Templeton, D. J., Quinones-Mateu, M. E., Offord, R. E., Arts, E. J. (2001). Mechanisms Involved in Stimulation of Human Immunodeficiency Virus Type 1 Replication by Aminooxypentane RANTES. J. Virol. 75: 8624-8638 [Abstract] [Full Text]  
  • Taylor, J. R. Jr., Kimbrell, K. C., Scoggins, R., Delaney, M., Wu, L., Camerini, D. (2001). Expression and Function of Chemokine Receptors on Human Thymocytes: Implications for Infection by Human Immunodeficiency Virus Type 1. J. Virol. 75: 8752-8760 [Abstract] [Full Text]  
  • Equils, O., Faure, E., Thomas, L., Bulut, Y., Trushin, S., Arditi, M. (2001). Bacterial Lipopolysaccharide Activates HIV Long Terminal Repeat Through Toll-Like Receptor 4. J. Immunol. 166: 2342-2347 [Abstract] [Full Text]  
  • Alfano, M., Vallanti, G., Biswas, P., Bovolenta, C., Vicenzi, E., Mantelli, B., Pushkarsky, T., Rappuoli, R., Lazzarin, A., Bukrinsky, M., Poli, G. (2001). The Binding Subunit of Pertussis Toxin Inhibits HIV Replication in Human Macrophages and Virus Expression in Chronically Infected Promonocytic U1 Cells. J. Immunol. 166: 1863-1870 [Abstract] [Full Text]  
  • Vicenzi, E., Alfano, M., Ghezzi, S., Gatti, A., Veglia, F., Lazzarin, A., Sozzani, S., Mantovani, A., Poli, G. (2000). Divergent regulation of HIV-1 replication in PBMC of infected individuals by CC chemokines: suppression by RANTES, MIP-1{alpha}, and MCP-3, and enhancement by MCP-1. J. Leukoc. Biol. 68: 405-412 [Abstract] [Full Text]  
  • Popik, W., Pitha, P. M. (2000). Inhibition of CD3/CD28-Mediated Activation of the MEK/ERK Signaling Pathway Represses Replication of X4 but Not R5 Human Immunodeficiency Virus Type 1 in Peripheral Blood CD4+ T Lymphocytes. J. Virol. 74: 2558-2566 [Abstract] [Full Text]  
  • Cota, M., Mengozzi, M., Vicenzi, E., Panina-Bordignon, P., Sinigaglia, F., Transidico, P., Sozzani, S., Mantovani, A., Poli, G. (2000). Selective inhibition of HIV replication in primary macrophages but not T lymphocytes by macrophage-derived chemokine. Proc. Natl. Acad. Sci. USA 97: 9162-9167 [Abstract] [Full Text]  
  • Mengozzi, M., Malipatlolla, M., De Rosa, S. C., Herzenberg, L. A., Herzenberg, L. A., Roederer, M. (2001). Naive CD4 T cells inhibit CD28-costimulated R5 HIV replication in memory CD4 T cells. Proc. Natl. Acad. Sci. USA 98: 11644-11649 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»