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Journal of Virology, September 1999, p. 7505-7514, Vol. 73, No. 9
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Translation Initiation Factor eIF4B Interacts with a Picornavirus Internal Ribosome Entry Site in both 48S and 80S Initiation Complexes Independently of Initiator AUG Location

Kerstin Ochs, René C. Rust,dagger and Michael Niepmann*

Institute of Biochemistry, D-35392 Giessen, Germany

Received 2 February 1999/Accepted 3 June 1999

Most eukaryotic initiation factors (eIFs) are required for internal translation initiation at the internal ribosome entry site (IRES) of picornaviruses. eIF4B is incorporated into ribosomal 48S initiation complexes with the IRES RNA of foot-and-mouth disease virus (FMDV). In contrast to the weak interaction of eIF4B with capped cellular mRNAs and its release upon entry of the ribosomal 60S subunit, eIF4B remains tightly associated with the FMDV IRES during formation of complete 80S ribosomes. Binding of eIF4B to the IRES is energy dependent, and binding of the small ribosomal subunit to the IRES requires the previous energy-dependent association of initiation factors with the IRES. The interaction of eIF4B with the IRES in 48S and 80S complexes is independent of the location of the initiator AUG and thus independent of the mechanism by which the small ribosomal subunit is placed at the actual start codon, either by direct internal ribosomal entry or by scanning. eIF4B does not greatly rearrange its binding to the IRES upon entry of the ribosomal subunits, and the interaction of eIF4B with the IRES is independent of the polypyrimidine tract-binding protein, which enhances FMDV translation.

* Corresponding author. Mailing address: Institute of Biochemistry, Friedrichstrasse 24, D-35392 Giessen, Germany. Phone: 49-641-99-47421. Fax: 49-641-99-47429. E-mail: michael.niepmann{at}biochemie.med.uni-giessen.de.

dagger Present address: Institute of Medical Microbiology, University of Basel, CH-4003 Basel, Switzerland.

Journal of Virology, September 1999, p. 7505-7514, Vol. 73, No. 9
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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