This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pastey, M. K.
Right arrow Articles by Graham, B. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pastey, M. K.
Right arrow Articles by Graham, B. S.

 Previous Article  |  Next Article 

Journal of Virology, September 1999, p. 7262-7270, Vol. 73, No. 9
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

RhoA Interacts with the Fusion Glycoprotein of Respiratory Syncytial Virus and Facilitates Virus-Induced Syncytium Formation

Manoj K. Pastey,1 James E. Crowe Jr.,2,3 and Barney S. Graham1,2,*

Departments of Medicine,1 Microbiology & Immunology,2 and Pediatrics,3 Vanderbilt University School of Medicine, Nashville, Tennessee 37232

Received 8 April 1999/Accepted 8 June 1999

The fusion glycoprotein (F) of respiratory syncytial virus (RSV), which mediates membrane fusion and virus entry, was shown to bind RhoA, a small GTPase, in yeast two-hybrid interaction studies. The interaction was confirmed in vivo by mammalian two-hybrid assay and in RSV-infected HEp-2 cells by coimmunoprecipitation. Furthermore, the interaction of F with RhoA was confirmed in vitro by enzyme-linked immunosorbent assay and biomolecular interaction analysis. Yeast two-hybrid interaction studies with various deletion mutants of F and with RhoA indicate that the key binding domains of these proteins are contained within, or overlap, amino acids 146 to 155 and 67 to 110, respectively. The biological significance of this interaction was studied in RSV-infected HEp-2 cells that were stably transfected to overexpress RhoA. There was a positive correlation between RhoA expression and RSV syncytium formation, indicating that RhoA can facilitate RSV-induced syncytium formation.

* Corresponding author. Mailing address: A-4103 MCN, Vanderbilt University School of Medicine, 1161 21st Ave. South, Nashville, TN 37232-2582. Phone: (615) 343-3717. Fax: (615) 322-8222. E-mail: Barney.Graham{at}mcmail.vanderbilt.edu.

Journal of Virology, September 1999, p. 7262-7270, Vol. 73, No. 9
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Batonick, M., Oomens, A. G. P., Wertz, G. W. (2008). Human Respiratory Syncytial Virus Glycoproteins Are Not Required for Apical Targeting and Release from Polarized Epithelial Cells. J. Virol. 82: 8664-8672 [Abstract] [Full Text]  
  • Eckardt-Michel, J., Lorek, M., Baxmann, D., Grunwald, T., Keil, G. M., Zimmer, G. (2008). The Fusion Protein of Respiratory Syncytial Virus Triggers p53-Dependent Apoptosis. J. Virol. 82: 3236-3249 [Abstract] [Full Text]  
  • Fernandes, L. B., Henry, P. J., Goldie, R. G. (2007). Review: Rho kinase as a therapeutic target in the treatment of asthma and chronic obstructive pulmonary disease. Ther Adv Respir Dis 1: 25-33 [Abstract]  
  • Crim, R. L., Audet, S. A., Feldman, S. A., Mostowski, H. S., Beeler, J. A. (2007). Identification of Linear Heparin-Binding Peptides Derived from Human Respiratory Syncytial Virus Fusion Glycoprotein That Inhibit Infectivity. J. Virol. 81: 261-271 [Abstract] [Full Text]  
  • Oomens, A. G. P., Wertz, G. W. (2004). trans-Complementation Allows Recovery of Human Respiratory Syncytial Viruses That Are Infectious but Deficient in Cell-to-Cell Transmission. J. Virol. 78: 9064-9072 [Abstract] [Full Text]  
  • Budge, P. J., Graham, B. S. (2004). Inhibition of respiratory syncytial virus by RhoA-derived peptides: implications for the development of improved antiviral agents targeting heparin-binding viruses. J Antimicrob Chemother 54: 299-302 [Abstract] [Full Text]  
  • Budge, P. J., Li, Y., Beeler, J. A., Graham, B. S. (2004). RhoA-Derived Peptide Dimers Share Mechanistic Properties with Other Polyanionic Inhibitors of Respiratory Syncytial Virus (RSV), Including Disruption of Viral Attachment and Dependence on RSV G. J. Virol. 78: 5015-5022 [Abstract] [Full Text]  
  • Easton, A. J., Domachowske, J. B., Rosenberg, H. F. (2004). Animal Pneumoviruses: Molecular Genetics and Pathogenesis. Clin. Microbiol. Rev. 17: 390-412 [Abstract] [Full Text]  
  • Budge, P. J., Lebowitz, J., Graham, B. S. (2003). Antiviral Activity of RhoA-Derived Peptides against Respiratory Syncytial Virus Is Dependent on Formation of Peptide Dimers. Antimicrob. Agents Chemother. 47: 3470-3477 [Abstract] [Full Text]  
  • McCurdy, L. H., Graham, B. S. (2003). Role of Plasma Membrane Lipid Microdomains in Respiratory Syncytial Virus Filament Formation. J. Virol. 77: 1747-1756 [Abstract] [Full Text]  
  • Hashimoto, K, Peebles, R S Jr, Sheller, J R, Jarzecka, K, Furlong, J, Mitchell, D B, Hartert, T V, Graham, B S (2002). Suppression of airway hyperresponsiveness induced by ovalbumin sensitisation and RSV infection with Y-27632, a Rho kinase inhibitor. Thorax 57: 524-527 [Abstract] [Full Text]  
  • Brown, G., Aitken, J., Rixon, H. W. McL., Sugrue, R. J. (2002). Caveolin-1 is incorporated into mature respiratory syncytial virus particles during virus assembly on the surface of virus-infected cells. J. Gen. Virol. 83: 611-621 [Abstract] [Full Text]  
  • McNamara, P. S, Smyth, R. L (2002). The pathogenesis of respiratory syncytial virus disease in childhood. Br Med Bull 61: 13-28 [Abstract] [Full Text]  
  • Gower, T. L., Graham, B. S. (2001). Antiviral Activity of Lovastatin against Respiratory Syncytial Virus In Vivo and In Vitro. Antimicrob. Agents Chemother. 45: 1231-1237 [Abstract] [Full Text]  
  • Feldman, S. A., Audet, S., Beeler, J. A. (2000). The Fusion Glycoprotein of Human Respiratory Syncytial Virus Facilitates Virus Attachment and Infectivity via an Interaction with Cellular Heparan Sulfate. J. Virol. 74: 6442-6447 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»