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Journal of Virology, September 1999, p. 7099-7107, Vol. 73, No. 9
0022-538X/99/$04.00+0

Respiratory Syncytial Virus G and/or SH Protein Alters Th1 Cytokines, Natural Killer Cells, and Neutrophils Responding to Pulmonary Infection in BALB/c Mice

Ralph A. Tripp,1,* Deborah Moore,1 Les Jones,1 Wayne Sullender,2 Jorn Winter,1 and Larry J. Anderson1

Division of Viral and Rickettsial Diseases, National Center of Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333,1 and Departments of Pediatrics and Microbiology, University of Alabama School of Medicine, Birmingham, Alabama 352332

Received 1 March 1999/Accepted 12 May 1999

BALB/c mice sensitized to vaccinia virus expressed G protein of respiratory syncytial virus (RSV) develop a Th2-type cytokine response and pulmonary eosinophilia when challenged with live RSV. In this study, BALB/c mice were immunized or challenged with an RSV mutant lacking the G and SH proteins or with DNA vaccines coding for RSV G or F protein. F or G protein DNA vaccines were capable of sensitizing for pulmonary eosinophilia. The absence of the G and/or SH protein in the infecting virus resulted in a consistent increase both in pulmonary natural killer cells and in gamma interferon and tumor necrosis factor expression, as well as, with primary infection, a variable increase in neutrophils and CD11b+ cells. The development of pulmonary eosinophilia in formalin-inactivated RSV-vaccinated mice required the presence of the G and/or SH protein in the challenge virus. These data show that G and/or SH protein has a marked impact on the inflammatory and innate immune response to RSV infection.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, 1600 Clifton Rd., MS G-09, Atlanta, GA 30333. Phone: (404) 639-3427. Fax: (404) 639-1307. E-mail: rgt3{at}cdc.gov.

Journal of Virology, September 1999, p. 7099-7107, Vol. 73, No. 9
0022-538X/99/$04.00+0


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