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Journal of Virology, August 1999, p. 7087-7092, Vol. 73, No. 8
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Interaction of Human Immunodeficiency Virus-Derived Vectors with Wild-Type Virus in Transduced Cells

Anatoly A. Bukovsky, Jin-Ping Song, and Luigi Naldini*

Cell Genesys, Foster City, California 94404

Received 12 October 1998/Accepted 15 April 1999

The interaction of human immunodeficiency virus (HIV)-derived vectors with wild-type virus was analyzed in transduced cells. Vector transcripts upregulated by infection had no measurable effect on HIV type 1 (HIV-1) expression but competed efficiently for encapsidation, inhibiting the infectivity and spread of HIV-1 in culture and leading to mobilization and recombination of the vector. These effects were abrogated with a self-inactivating vector.

* Corresponding author. Present address: Laboratory for Gene Transfer and Therapy, IRCC Institute for Cancer Research, University of Torino Medical School, S.P. 142, 10060 Candiolo (Torino), Italy. Phone: 39-011-993.3226. Fax: 39-011-993.3225. E-mail: lnaldini{at}ircc.unito.it.

Journal of Virology, August 1999, p. 7087-7092, Vol. 73, No. 8
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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