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Journal of Virology, August 1999, p. 7035-7038, Vol. 73, No. 8
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Human Parainfluenza Virus Type 1 Matrix and Nucleoprotein Genes Transiently Expressed in Mammalian Cells Induce the Release of Virus-Like Particles Containing Nucleocapsid-Like Structures

Elizabeth C. Coronel, K. Gopal Murti, Toru Takimoto, and Allen Portner*

Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105

Received 25 January 1999/Accepted 4 May 1999

The matrix (M) protein plays an essential role in the assembly and budding of some enveloped RNA viruses. We expressed the human parainfluenza virus type 1 (hPIV-1) M and/or NP genes into 293T cells using the mammalian expression vector pCAGGS. Biochemical and electron microscopic analyses of transfected cells showed that the M protein alone can induce the budding of virus-like particles (vesicles) from the plasma membrane and that the NP protein can assemble into intracellular nucleocapsid-like (NC-like) structures. Furthermore, the coexpression of both the M and NP genes resulted in the production of vesicles enclosing NC-like structures, suggesting that the hPIV-1 M protein has the intrinsic ability to induce membrane vesiculation and to incorporate NC-like structures into these budding vesicles.

* Corresponding author. Mailing address: Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, 332 N. Lauderdale, Memphis, TN 38105. Phone: (901) 495-3400. Fax: (901) 523-2622. E-mail: allen.portner{at}stjude.org.

Journal of Virology, August 1999, p. 7035-7038, Vol. 73, No. 8
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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