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Journal of Virology, August 1999, p. 6903-6915, Vol. 73, No. 8
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
A Recombinant Measles Vaccine Virus Expressing Wild-Type
Glycoproteins: Consequences for Viral Spread and Cell
Tropism
Ian C. D.
Johnston,
V.
ter Meulen,
Jürgen
Schneider-Schaulies, and
Sibylle
Schneider-Schaulies*
Institute of Virology and Immunobiology,
University of Würzburg, 97078 Würzburg, Germany
Received 2 February 1999/Accepted 11 May 1999
Wild-type, lymphotropic strains of measles virus (MV) and tissue
culture-adapted MV vaccine strains possess different cell tropisms.
This observation has led to attempts to identify the viral receptors
and to characterize the functions of the MV glycoproteins. We have
functionally analyzed the interactions of MV hemagglutinin (H) and
fusion (F) proteins of vaccine (Edmonston) and wild-type (WTF) strains
in different combinations in transfected cells. Cell-cell fusion occurs
when both Edmonston F and H proteins are expressed in HeLa or Vero
cells. The expression of WTF glycoproteins in HeLa cells did not result
in syncytia, yet they fused efficiently with cells of lymphocytic
origin. To further investigate the role of the MV glycoproteins in
virus cell entry and also the role of other viral proteins in cell
tropism, we generated recombinant vaccine MVs containing one or both
glycoproteins from WTF. These viruses were viable and grew similarly in
lymphocytic cells. Recombinant viruses expressing the WTFH protein
showed a restricted spread in HeLa cells but spread efficiently in Vero
cells. Parental WTF remained restricted in both cell types. Therefore,
not only differential receptor usage but also other cell-specific
factors are important in determining MV cell tropism.
*
Corresponding author. Mailing address: Institute of
Virology and Immunobiology, University of Würzburg,
Versbacher Str. 7, 97078 Würzburg, Germany. Phone:
49-931-201-3895. Fax: 49-931-201-3934. E-mail:
s-s-s{at}vim.uni-wuerzburg.de.

Present address: Miltenyi Biotech GmbH, 51429 Bergisch Gladbach,
Germany.
Journal of Virology, August 1999, p. 6903-6915, Vol. 73, No. 8
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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