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Journal of Virology, August 1999, p. 6566-6572, Vol. 73, No. 8
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Regulation of Epstein-Barr Virus Promoters in Oral Epithelial Cells and Lymphocytes

Laurel A. Lagenaur1,dagger and Joel M. Palefsky1,2,*

Departments of Stomatology1 and Laboratory Medicine,2 University of California, San Francisco, California

Received 26 January 1999/Accepted 12 May 1999

Hairy leukoplakia (HL) is a proliferative lesion of the tongue that supports abundant Epstein-Barr virus (EBV) replication. Previous work showed high-level expression of the EBV BMRF2 gene in HL. To characterize the regulation of BMRF2 expression in HL, we mapped the 5' ends of the BMRF1 and BMRF2 transcripts and showed that BMRF2 is expressed from a novel internal promoter within the BMRF1 coding region. Mechanisms of BMRF2 regulation were compared in oral epithelial cells and B lymphocytes, as were those of BMRF1 and BDLF3, early and late EBV transcripts, respectively, that are also known to be expressed in HL. Basal activity of the putative BMRF2 promoter was 10-fold higher in HSC-3 epithelial cells than in B lymphocytes. The BMRF2 and the BDLF3 promoters were responsive to induction by phorbol ester, but unlike the BMRF1 promoter, they were not responsive to BZLF1 transactivation. By mutational analysis, the major activity of the BMRF2 promoter mapped to a 50-bp region, which includes a TATA-like element and a GC box. The BMRF2 promoter may be regulated differentially from the BMRF1 promoter and more closely resembles that of BDLF3. This novel BMRF2 promoter likely belongs to a class of viral promoters that is more responsive to mechanisms known to induce epithelial cell differentiation, consistent with its high level of expression in HL.

* Corresponding author. Mailing address: Department of Stomatology, 521 Parnassus, Box 0512, University of California, San Francisco, CA 94143-0512. Phone: (415) 476-1574. Fax: (415) 476-4204. E-mail: joelp{at}pangloss.ucsf.edu.

dagger Present address: Department of Infectious Diseases, Stanford University, Stanford, CA 94305-5428.

Journal of Virology, August 1999, p. 6566-6572, Vol. 73, No. 8
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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