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Journal of Virology, August 1999, p. 6387-6393, Vol. 73, No. 8
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Proteolytic Activity, the Carboxy Terminus of Gag, and the Primer
Binding Site Are Not Required for Pol Incorporation into Foamy
Virus Particles
David N.
Baldwin and
Maxine L.
Linial*
Division of Basic Sciences, Fred Hutchinson
Cancer Research Center, Seattle, Washington 98109, and Department of
Microbiology, University of Washington, Seattle, Washington 98195
Received 3 March 1999/Accepted 4 May 1999
Human foamy virus (HFV) is the prototype member of the
spumaviruses. While similar in genomic organization to other complex retroviruses, foamy viruses share several features with their more
distant relatives, the hepadnaviruses such as human hepatitis B virus
(HBV). Both HFV and HBV express their Pol proteins independently from
the structural proteins. However unlike HBV, Pol is not required for
assembly of HFV core particles or for packaging of viral RNA. These
results suggest that the assembly of Pol into HFV particles must occur
by a mechanism different from those used by retroviruses and
hepadnaviruses. We have examined possible mechanisms for HFV Pol
incorporation, including the role of proteolysis in assembly of Pol and
the role of initiation of reverse transcription. We have found that
proteolytic activity is not required for Pol incorporation. p4 Gag and
the residues immediately upstream of the cleavage site in Gag are also
not important. Deletion of the primer binding site had no effect on
assembly, ruling out early steps of reverse transcription in the
process of Pol incorporation.
*
Corresponding author. Mailing address: Division of
Basic Sciences, Fred Hutchinson Cancer Research Center, A3-015, 1100 Fairview Ave. N., P.O. Box 19024, Seattle, WA 98109-1024. Phone: (206) 667-4442. Fax: (206) 667-5939. E-mail: mlinial{at}fhcrc.org.
Journal of Virology, August 1999, p. 6387-6393, Vol. 73, No. 8
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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